Effects of different storage solutions on renal ischemia tolerance after kidney transplantation in mice.
Effects of different storage solutions on renal ischemia tolerance after kidney transplantation in mice.
复制标题
DOI：
10.1152/ajprenal.00475.2017
复制
发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Lei Wang;Jin Wei;Shan Jiang;Huihui Li;L. Fu;Jie Zhang;Ruisheng Liu
中科院分区：
文献类型：
--
作者：
Lei Wang;Jin Wei;Shan Jiang;Huihui Li;L. Fu;Jie Zhang;Ruisheng Liu
储存是肾移植（KTX）中移植物保存的最普遍方法。各种保存液的保护作用已在临床试验和实验动物模型中得到广泛研究。然而，很少有研究检查了不同的保存溶液对小鼠KTX移植物功能的影响;此外，尚未评估移植移植物对进一步损伤的耐受性，这是本研究的目的。我们分三组进行小鼠KTX，供体肾保存在不同的溶液中60分钟：生理盐水，小鼠血清，和威斯康星州大学（UW）的解决方案。通过肾损伤指标和肾小球滤过率（GFR）评价移植肾功能。在移植后第一周内，保存在UW溶液中的移植物表现出更好的功能，反映在GFR中的血浆肌酐水平分别比血清和盐水组低50%和70%以及血浆肌酐水平高30%和55%。为了检测移植肾功能对额外损伤的反应，我们在KTX后4周通过夹闭肾蒂18分钟诱导缺血再灌注损伤（IRI）。我们发现，在UW溶液中保存的移植物表现出约30%和20%的肾损伤标志物和组织学评估比其他两种保存液的损伤。UW液对小鼠移植肾有较好的保护作用。建议将UW溶液用于小鼠KTX，以减少手术期间的混杂因素，如IRI。
storage is the most prevalent method for graft preservation in kidney transplantation (KTX). The protective effects of various preservation solutions have been studied extensively in both clinical trials and experimental animal models. However, a paucity of studies have examined the effect of different preservation solutions on graft function in mouse KTX; in addition, the tolerance of the transplanted grafts to further insult has not been evaluated, which was the objective of the present study. We performed mouse KTX in three groups, with the donor kidneys preserved in different solutions for 60 min: saline, mouse serum, and University of Wisconsin (UW) solution. The graft functions were assessed by kidney injury markers and glomerular filtration rate (GFR). The grafts that were preserved in UW solution exhibited better functions, reflected by 50 and 70% lower plasma creatinine levels as well as 30 and 55% higher plasma creatinine levels in GFR than serum and saline groups, respectively, during the first week after transplants. To examine the graft function in response to additional insult, we induced ischemia-reperfusion injury (IRI) by clamping the renal pedicle for 18 min at 4 wk after KTX. We found that the grafts preserved in UW solution exhibited ~30 and 20% less injury assessed by kidney injury markers and histology than in other two preservation solutions. Taken together, our results demonstrated that UW solution exhibited a better protective effect in transplanted renal grafts in mice. UW solution is recommended for use in mouse KTX for reducing confounding factors such as IRI during surgery.