O-Antigen-Negative Salmonella enterica Serovar Typhimurium Is Attenuated in Intestinal Colonization but Elicits Colitis in Streptomycin-Treated Mice
O-Antigen-Negative Salmonella enterica Serovar Typhimurium Is Attenuated in Intestinal Colonization but Elicits Colitis in Streptomycin-Treated Mice
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DOI：
10.1128/iai.01537-08
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发表时间：
2009-06-01
影响因子：
3.1
通讯作者：
Hardt, Wolf-Dietrich
中科院分区：
文献类型：
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作者：
Ilg, Karin;Endt, Kathrin;Hardt, Wolf-Dietrich
脂多糖（LPS）是肠道沙门氏菌亚种1血清型鼠伤寒沙门氏菌（鼠伤寒血清型）外膜的主要成分和重要的毒力因子。为了评估LPS在链霉素处理的小鼠中引发肠道炎症的作用，我们通过缺失wbaP基因构建了O抗原缺陷型血清型鼠伤寒菌株。所得菌株对人补体活性和抗微生物肽模拟物多粘菌素B高度敏感。此外，它在竞争性小鼠感染实验中显示出运动性和减毒表型的严重缺陷，其中Delta wbaP菌株（SKI12）直接与野生型沙门氏菌进行比较。然而，Delta wbaP菌株（SKI12）在体外有效地侵入HeLa细胞，并在链霉素预处理的小鼠中引起急性肠道炎症。我们的实验证明，完整的LPS的存在不是必要的体外入侵或触发急性结肠炎。
Lipopolysaccharide (LPS) is a major constituent of the outer membrane and an important virulence factor of Salmonella enterica subspecies 1 serovar Typhimurium (serovar Typhimurium). To evaluate the role of LPS in eliciting intestinal inflammation in streptomycin-treated mice, we constructed an O-antigen-deficient serovar Typhimurium strain through deletion of the wbaP gene. The resulting strain was highly susceptible to human complement activity and the antimicrobial peptide mimic polymyxin B. Furthermore, it showed a severe defect in motility and an attenuated phenotype in a competitive mouse infection experiment, where the Delta wbaP strain (SKI12) was directly compared to wild-type Salmonella. Nevertheless, the Delta wbaP strain (SKI12) efficiently invaded HeLa cells in vitro and elicited acute intestinal inflammation in streptomycin-pretreated mice. Our experiments prove that the presence of complete LPS is not essential for in vitro invasion or for triggering acute colitis.