Ascorbic acid modulates the production of fibronectin and laminin by cells from an eye tissue-trabecular meshwork.
Ascorbic acid modulates the production of fibronectin and laminin by cells from an eye tissue-trabecular meshwork.
复制标题
抗坏血酸调节眼组织-小梁网细胞产生纤连蛋白和层粘连蛋白。
DOI：
10.1016/0014-4827(90)90117-s
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发表时间：
1990
影响因子：
3.7
通讯作者：
Chang,IL
中科院分区：
文献类型：
--
作者：
Yue,BY;Higginbotham,EJ;Chang,IL
抗坏血酸是眼的房水中的重要成分，小梁网细胞浸没在其中。采用免疫组化染色和Western blot分析，观察抗坏血酸对体外培养的牛眼小梁细胞分泌细胞外基质的影响。结果发现，当用100、250和500 μg/ml的抗坏血酸处理培养物时，产生的纤连蛋白和层粘连蛋白的量增加。用于定量的酶联免疫吸附测定进一步表明，抗坏血酸处理的培养物中的纤连蛋白和层粘连蛋白水平高于对照培养物中的水平。这些结果表明，抗坏血酸促进纤维连接蛋白和层粘连蛋白的生产，并建议，这种物质可能是一个因素，调节基底层组装在小梁网。
Ascorbic acid is a significant component in the aqueous humor of the eye, in which trabecular meshwork cells are immersed. Using immunostaining and Western blot analysis, the effects of ascorbic acid on the extracellular matrix production by cultured bovine trabecular meshwork cells were examined. It was found that, when the cultures were treated with 100, 250, and 500 μg/ml of ascorbic acid, the amount of fibronectin and laminin produced was enhanced. An enzyme-linked immunosorbent assay for quantitation further showed that the fibronectin and laminin levels in ascorbate-treated cultures were higher than those found in control cultures. These results indicate that ascorbic acid promotes fibronectin and laminin production and suggest that this substance may be one of the factors modulating basal lamina assembly in the trabecular meshwork.