Ventral neural tube cells differentiate into craniofacial skeletal muscles.
Ventral neural tube cells differentiate into craniofacial skeletal muscles.
复制标题
腹神经管细胞分化为颅面骨骼肌。
DOI：
10.1006/bbrc.1998.9715
复制
发表时间：
1998
影响因子：
3.1
通讯作者：
Ali,MM
中科院分区：
文献类型：
--
作者：
Sohal,GS;Ali,AA;Ali,MM
颅面骨骼肌细胞被认为是从中胚层发育而来的。一群腹侧神经管细胞最近已被证明迁移出后脑和填充在鸡胚颅面间充质。由于骨骼肌细胞是从这种间充质发育而来，我们试图确定迁移的神经管细胞是否有助于它们的发育。在胚胎第2天，用含有LacZ基因的复制缺陷型逆转录病毒载体标记鸡胚后脑中的神经管细胞，LacZ基因为后代提供了永久性标记。在第7天，处理胚胎以检测标记的细胞。标记细胞可见于颅面骨骼肌。通过使用肌肉特异性标记物，证实标记的细胞是骨骼肌细胞。因此，一些肌肉细胞来自于后脑的腹侧神经管细胞。
Craniofacial skeletal muscle cells are believed to develop from mesoderm. A population of ventral neural tube cells has recently been shown to migrate out of the hindbrain and populate the craniofacial mesenchyme in chick embryos. Since skeletal muscle cells develop from this mesenchyme, we sought to determine if the emigrated neural tube cells contributed to their development. Ventral neural tube cells in the hindbrain of chick embryos were labeled on embryonic day 2 with replication-deficient retroviral vectors containing the gene LacZ, which provides a permanent marker for the progeny. On day 7 embryos were processed for the detection of labeled cells. Labeled cells were seen in craniofacial skeletal muscles. By using muscle-specific markers, the labeled cells were confirmed to be skeletal muscle cells. Thus, some muscle cells are derived from the ventral neural tube cells of the hindbrain.