Differential expression of GAPDH and β-actin in growing collateral arteries
Differential expression of GAPDH and β-actin in growing collateral arteries
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DOI：
10.1023/a:1016166127465
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发表时间：
2002-07-01
影响因子：
4.3
通讯作者：
Schaper, W
中科院分区：
文献类型：
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作者：
Deindl, E;Boengler, K;Schaper, W
看家基因如甘油醛-3-磷酸脱氢酶(GAPDH)和β-肌动蛋白经常被用作定量RNA分析的内标。在我们的研究中，我们分析了GAPDH和β-肌动蛋白以及18S rRNA和Poly(A)(+)RNA在与缺血无关的兔动脉生成模型中的相对表达水平。管家基因的相对定量显示，在血管生长的前24小时，β-肌动蛋白和GAPDHmRNA显著上调。在第3天，我们的结果显示，β-肌动蛋白mRNA上调更强(140%)，而GAPDHmRNA显着下调(对照组的50%)。然而，与Poly(A)+RNA相比，18S rRNA在相同时期仅显示出轻微的变化。根据这些结果，我们得出结论，18S rRNA而不是GAPDH或β-肌动蛋白mRNA是在生长的血管中细胞增殖条件下相对定量基因表达的合适的内参照。
Housekeeping genes like glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and beta-actin are often used as internal standards for quantitative RNA analysis. In our study we analyzed the relative expression level of GAPDH and beta-actin as well as of the 18S rRNA and the Poly (A)(+) RNA in growing collateral arteries in a rabbit model of arteriogenesis which is not associated with ischemia. Relative quantitation of the housekeeping genes displayed a significant upregulation of the beta-actin- and GAPDH mRNA during the first 24 h of vessel growth. For day 3 our results revealed an even stronger upregulation of the beta-actin mRNA (140%) but a significant downregulation of the GAPDH mRNA (50% of control). The 18S rRNA, however, showed for the same periods only minor alterations compared to the Poly ( A) + RNA. From these results we conclude that the 18S rRNA, but not the GAPDH- or beta-actin mRNA is an appropriate internal control for relative quantitation of gene expression under conditions of cell proliferation in growing vessels.