Different sensitivities to calcineurin inhibitors between B cells responding to blood group A and B antigens
Different sensitivities to calcineurin inhibitors between B cells responding to blood group A and B antigens
复制标题
对 A 型和 B 型抗原的 B 细胞对钙调神经磷酸酶抑制剂的敏感性不同
DOI：
10.1111/voxs.12233
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ohdan H.
中科院分区：
文献类型：
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作者：
Yamashita M;Tanaka Y;Tazawa H;Sakai H;Ohdan H.
背景和目的我们评估了A -半乳糖转移酶缺陷（GalTÀ/À）小鼠中具有人血型A和B决定因子受体的B细胞的表型和功能差异。材料与方法GalTÀ/À小鼠用人血A或B型红细胞（rbc）免疫，测定其血清中抗A或B抗体（Abs）的含量。使用合成的A和B决定因子鉴定具有A组和B组表位受体的B细胞。结果A/ b -红细胞免疫GalTÀ/À小鼠血清中抗A、抗b抗体水平明显升高。携带识别B表位受体的B细胞表现为CD5dim/À CD11b+ B-1b-ell表型，而携带识别A表位受体的B细胞表现为CD5+CD11b+ B-1a细胞表型。髓样分化因子88 （MyD88）水平在具有抗B受体的B细胞中升高，表明toll样受体（TLR）信号传导的作用，而在具有抗a受体的B细胞中未检测到这种升高。钙调磷酸酶抑制剂（CNI）可阻止免疫后抗a抗体水平升高，但不影响抗b抗体水平。结论A血型B细胞和B血型B细胞对CNIs的敏感性不同。
Background and Objectives We evaluated the phenotypic and functional distinction between B cells with receptors for human blood group A and B determinants in a-galactosyltransferase-deficient (GalTÀ/À) mice. Materials and Methods GalTÀ/À mice were immunized with human blood group A or B red blood cells (RBCs), and the anti-A or B anti-bodies (Abs) were determined in their sera. B cells with receptors for group A and B epitopes were identified by the use of synthetic A and B determinants. Results The anti-A and anti-B Ab levels were comparably elevated in the sera of GalTÀ/À mice after the immunization with A/B-RBCs. B cells bearing receptors recognizing B epitopes exhibited the CD5dim/À CD11b+ B-1b-ell phenotype, whereas those with receptors for A epitopes showed the CD5+CD11b+ B-1a-cell phenotype. Myeloid differentiation factor 88 (MyD88) levels increased in B cells with anti-B receptors, indicating a contribution of Toll-like receptor (TLR) signalling, whereas no such elevation was detected in B cells with anti-A receptors. Calcineurin inhibitor (CNI) treatment prevented the elevation of anti-A Ab levels after immunization, but did not affect anti-B Ab levels. Conclusions These findings indicate different sensitivities of B cells with blood group A and B antigens in the response to CNIs.