Amperometric glucose biosensor based on glucose oxidase immobilized over chitosan nanoparticles from gladius of Uroteuthis duvauceli
Amperometric glucose biosensor based on glucose oxidase immobilized over chitosan nanoparticles from gladius of Uroteuthis duvauceli
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DOI：
10.1016/j.snb.2015.03.110
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发表时间：
2015-08-01
影响因子：
8.4
通讯作者：
Kim, Byung Chul
中科院分区：
文献类型：
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作者：
Anusha, J. R.;Raj, C. Justin;Kim, Byung Chul
将葡萄糖氧化酶（GOx）固定在壳聚糖纳米颗粒（CSNPs）上，制成电流型葡萄糖生物传感器。以杜氏尾乌贼骨为原料，采用离子凝胶法制备壳聚糖纳米粒。利用透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）对CSNPs的形貌和功能基团进行了表征。对比分析了CSNPs修饰电极（Au/CSNPs/GOx）和商品化壳聚糖微粒修饰电极（Au/CS/GOx）的电化学葡萄糖传感性能。利用扫描电子显微镜（SEM）观察了裸Au、Au/CSNPs和Au/CS电极的表面形貌。循环伏安法测试结果表明，在氧气存在下，两种电极对葡萄糖的检测都表现出很高的电催化能力。该传感器对葡萄糖具有良好的安培响应，线性检测范围为0.001 - 1 mM，
Amperometric glucose biosensor was fabricated by immobilizing glucose oxidase (GOx) onto chitosan nanoparticles (CSNPs) in gold electrode. The chitosan nanoparticles were synthesized from gladius of squid, Uroteuthis duvauceli by ionic gelation process. The morphology and functional groups of CSNPs were characterized using transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) respectively. The electrochemical glucose sensing properties of CSNPs based electrode (Au/CSNPs/GOx) was analyzed relatively with commercial chitosan particles utilized electrode(Au/CS/GOx). The surface morphology of bare Au, Au/CSNPs and Au/CS electrodes were observed using scanning electron microscopy (SEM). The cyclic voltammetry measurements revealed that both the electrodes exhibit high electrocatalytic ability towards glucose detection in the presence of oxygen. The developed biosensors possessed good amperometric response towards glucose with linear detectionrange from 0.001 to 1 mM with