rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion.
rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion.
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rSec6 和 rSec8，分泌所必需的酵母蛋白的哺乳动物同源物。
DOI：
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发表时间：
1995
影响因子：
11.1
通讯作者：
Richard H. Scheller
中科院分区：
文献类型：
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作者：
A. E. Ting;Christopher D. Hazuka;Shu;Mark D. Kirk;Mark D. Kirk;Andrew J. Bean;Richard H. Scheller
神经传递所必需的许多分子与参与酵母分泌途径的高尔基体至质膜阶段的蛋白质同源。已知的15个基因是必不可少的后期阶段的囊泡运输酵母，7没有确定的哺乳动物同源物。这些包括酵母SEC 6、SEC 8和SEC 15基因，其产物是与GTP结合蛋白Sec 4p相互作用的19.5S颗粒的成分。在这里，我们报告的序列rSec 6和rSec 8，大鼠的同源物Sec 6p和Sec 8 p。预测rSec 6 cDNA编码与Sec 6p具有22%氨基酸同一性的87-kDa蛋白，并且预测rSec 8 cDNA编码与Sec 8 p具有20%同一性的110-kDa蛋白。北方印迹分析表明rSec 6和rSec 8在相似的组织中表达。免疫检测显示rSec 8是脑中可溶性17 S颗粒的一部分。COS细胞共转染研究表明，rSec 8与GTP结合蛋白Rab 3a和syntaxin 1a共定位，这两种蛋白质参与突触前末端的突触囊泡对接和融合。这些数据表明，rSec 8是一个高分子量的复合物，可能参与调节囊泡对接和融合在大脑中的组成部分。
Many of the molecules necessary for neurotransmission are homologous to proteins involved in the Golgi-to-plasma membrane stage of the yeast secretory pathway. Of 15 genes known to be essential for the later stages of vesicle trafficking in yeast, 7 have no identified mammalian homologs. These include the yeast SEC6, SEC8, and SEC15 genes, whose products are constituents of a 19.5S particle that interacts with the GTP-binding protein Sec4p. Here we report the sequences of rSec6 and rSec8, rat homologs of Sec6p and Sec8p. The rSec6 cDNA is predicted to encode an 87-kDa protein with 22% amino acid identity to Sec6p, and the rSec8 cDNA is predicted to encode a 110-kDa protein which is 20% identical to Sec8p. Northern blot analysis indicates that rSec6 and rSec8 are expressed in similar tissues. Immunodetection reveals that rSec8 is part of a soluble 17S particle in brain. COS cell cotransfection studies demonstrate that rSec8 colocalizes with the GTP-binding protein Rab3a and syntaxin 1a, two proteins involved in synaptic vesicle docking and fusion at the presynaptic terminal. These data suggest that rSec8 is a component of a high molecular weight complex which may participate in the regulation of vesicle docking and fusion in brain.