Cytokinesis of neuroepithelial cells can divide their basal process before anaphase
Cytokinesis of neuroepithelial cells can divide their basal process before anaphase
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DOI：
10.1038/emboj.2008.227
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发表时间：
2008-12-03
期刊：
影响因子：
11.4
通讯作者：
Huttner, Wieland B.
中科院分区：
文献类型：
--
作者：
Kosodo, Yoichi;Toida, Kazunori;Huttner, Wieland B.
神经上皮（NE）细胞是脊椎动物中枢神经系统的主要干细胞和祖细胞，具有高度极化和伸长的特性。它们保留延伸至基底层的基底突，同时在心室区的顶侧进行有丝分裂。通过共聚焦显微镜、电子显微镜和延时成像研究胚胎小鼠、鸡和斑马鱼中枢神经系统中的NE细胞，我们发现这些细胞的基底突起在M期可以分裂。分裂发生在基部到顶端的方向，其次是继承的过程中的一个或两个子细胞。一个集群的苯胺，胞质分裂机器的一个重要组成部分，出现在远端的基底进程在前期，并被发现与F-肌动蛋白在分叉点，在增殖和神经原性NE细胞共定位。在分裂后期开始之前，基部突起中的GFP-茴香素从顶端移动到细胞体，然后在分裂末期从基部到顶端侵入分裂沟。M期NE细胞基突的分裂对卵裂面的方向以及有丝分裂和胞质分裂之间的关系具有重要意义。
Neuroepithelial (NE) cells, the primary stem and progenitor cells of the vertebrate central nervous system, are highly polarized and elongated. They retain a basal process extending to the basal lamina, while undergoing mitosis at the apical side of the ventricular zone. By studying NE cells in the embryonic mouse, chick and zebrafish central nervous system using confocal microscopy, electron microscopy and time-lapse imaging, we show here that the basal process of these cells can split during M phase. Splitting occurred in the basal-to-apical direction and was followed by inheritance of the processes by either one or both daughter cells. A cluster of anillin, an essential component of the cytokinesis machinery, appeared at the distal end of the basal process in prophase and was found to colocalize with F-actin at bifurcation sites, in both proliferative and neurogenic NE cells. GFP-anillin in the basal process moved apically to the cell body prior to anaphase onset, followed by basal-to-apical ingression of the cleavage furrow in telophase. The splitting of the basal process of M-phase NE cells has implications for cleavage plane orientation and the relationship between mitosis and cytokinesis.