Dietary exposure to bisphenol A affects memory function and neuroimmune biomarkers in allergic asthmatic mice
Dietary exposure to bisphenol A affects memory function and neuroimmune biomarkers in allergic asthmatic mice
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DOI：
10.1002/jat.4143
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发表时间：
2021-01-20
影响因子：
3.3
通讯作者：
Takano, Hirohisa
中科院分区：
文献类型：
--
作者：
Win-Shwe, Tin-Tin;Yanagisawa, Rie;Takano, Hirohisa
双酚A（BPA）是聚碳酸酯和环氧树脂的原料。用于各种家用电器、电子设备、办公自动化设备、医疗设备、移动的电话、汽车涂料、罐头内表面涂层、土木工程和建筑用粘合剂等。BPA是一种众所周知的内分泌干扰化学品，据报道，BPA对神经和免疫系统有不良影响。然而，BPA诱导的记忆障碍和过敏性哮喘受试者的神经免疫生物标志物的变化尚不清楚。我们的目的是研究BPA的饮食暴露对脑功能和生物标志物的影响，使用过敏性哮喘小鼠模型。5周龄雄性C3 H/HeJSlc小鼠从5至11周龄喂食两个剂量的含有BPA [0.901，9.01 μ g/kg/天]的饲料，并每2周通过腹膜内滴注给予卵清蛋白（OVA）。记忆功能通过一种新的物体识别测试来确定。采用实时荧光定量聚合酶链反应（RT-PCR）技术，对海马区记忆相关基因和免疫标志物进行研究。在这项研究中，受损的新物体识别发生在BPA暴露的小鼠在过敏原的存在。此外，在BPA暴露的过敏性哮喘小鼠中观察到海马中神经免疫生物标志物如N-甲基-D-天冬氨酸受体、肿瘤坏死因子-α、离子化钙结合衔接分子-1、环氧合酶-2和血红素加氧酶-1的表达水平上调。这些发现表明，BPA暴露可以诱导神经炎症，并引发过敏性哮喘小鼠记忆功能的损害。我们的研究表明，饮食中暴露于BPA可能会影响更高的大脑功能，通过调节神经免疫生物标志物在过敏性哮喘患者。
Bisphenol A (BPA) is a raw material of polycarbonate and epoxy resin. It is used for various household electrical appliances, electronic equipment, office automation equipment, medical equipment, mobile phones, paints for automobiles, internal surface coating of cans, and adhesives for civil engineering and construction. BPA is a well-known endocrine-disrupting chemical, and it was reported that BPA has an adverse effect on the nervous and immune systems. However, BPA-induced memory impairment and changes in neuroimmune biomarkers in the allergic asthmatic subject are not known yet. We aim to investigate the dietary exposure effect of BPA on brain function and biomarkers using allergic an asthmatic mouse model. Five-week-old male C3H/HeJSlc mice were fed two doses of BPA [0.901, 9.01 mu g/kg/day] contained chow diet from 5 to 11 weeks old and ovalbumin (OVA) was given by intratracheal instillation every 2 weeks. Memory function was determined by a novel object recognition test. Genes related to memory and immune markers in the hippocampus were investigated with the real-time polymerase chain reaction (RT-PCR) method. In this study, impaired novel object recognition occurred in BPA-exposed mice in the presence of an allergen. Moreover, upregulation of expression level of neuroimmune biomarkers such as N-methyl-D-aspartate receptor, tumor necrosis factor-alpha, ionized calcium-binding adapter molecule-1, cyclooxygenase-2, and heme oxygenase-1 in the hippocampus was observed in BPA-exposed allergic asthmatic mice. These findings show that BPA exposure can induce neuroinflammation and which triggers impairment of memory function in mice with allergic asthma. Our study indicated that dietary exposure to BPA may affect higher brain functions by modulating neuroimmune biomarkers in allergic asthmatic subjects.