3 MONOCLONAL-ANTIBODIES DEFINING DISTINCT DIFFERENTIATION ANTIGENS ASSOCIATED WITH DIFFERENT HIGH MOLECULAR-WEIGHT POLYPEPTIDES ON THE SURFACE OF HUMAN EMBRYONAL CARCINOMA-CELLS
3 MONOCLONAL-ANTIBODIES DEFINING DISTINCT DIFFERENTIATION ANTIGENS ASSOCIATED WITH DIFFERENT HIGH MOLECULAR-WEIGHT POLYPEPTIDES ON THE SURFACE OF HUMAN EMBRYONAL CARCINOMA-CELLS
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DOI：
10.1089/hyb.1984.3.347
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
AVNER, P
中科院分区：
文献类型：
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作者：
ANDREWS, PW;BANTING, G;AVNER, P
两个单克隆抗体（TRA-1-60和TRA-1-81）识别不同的细胞表面抗原的人胚胎癌（EC）细胞的生产和特点。这些抗体在间接放射免疫测定（RIA）和免疫荧光（IF）测定中与未分化的人EC细胞强烈反应，但仅与来自体外或异种移植肿瘤中分化的多能EC细胞的细胞弱反应或根本不反应，也不与也不表达人EC细胞的典型特征的其它生殖细胞肿瘤细胞系反应。它们不与鼠畸胎瘤细胞系反应。对其他人肿瘤细胞系和正常人组织的调查揭示，这些抗体识别的分子不限于人EC细胞，而是交叉反应表位出现在几种肿瘤和正常组织上，尽管每种抗体的解剖模式不同。两种抗体均免疫沉淀主要多肽（表观Mff约为100）。240，000）和次要多肽（表观Mff. 415，000），在这方面类似于另一种单克隆抗体8- 7 D。顺序免疫沉淀显示，3种抗体中的每一种与稍微不同MW的不同分子反应。由本发明抗体定义的表位不同于由已描述的其它人EC细胞特异性单克隆抗体识别的表位，并提供了用于研究多能人EC细胞分化的新标志物。
Two monoclonal antibodies (TRA-1-60 and TRA-1-81) recognizing distinct cell surface antigens on human embryonal carcinoma (EC) cells were produced and characterized. These antibodies reacted strongly with undifferentiated human EC cells in indirect radioimmunoassays (RIA) and immunofluorescence (IF) assays, but only weakly or not at all with cells derived from pluripotent EC cells differentiating in vitro or in xenograft tumors, nor with other germ cell tumor cell lines that did not also express the typical features of human EC cells. They did not react with murine teratocarcinoma cell lines. A survey of other human tumor cell lines and normal human tissues disclosed that molecules recognized by these antibodies are not confined to human EC cells but that cross-reacting epitopes appear on several neoplastic and normal tissues, although in a different anatomical pattern for each antibody. Both antibodies immunoprecipitated a major polypeptide (apparent MW .apprx. 240,000) and a minor polypeptide (apparent MW .apprx. 415,000) from lysates of 125I surface-labeled human EC cells, in this respect resembling another monoclonal antibody, 8-7D. Sequential immunoprecipitation revealed that each of the 3 antibodies reacted with different molecules of slightly different MW. The epitopes defined by the present antibodies differ from those recognized by the other human EC cell-specific monooclonal antibodies that have been described and provide new markers for studying the differentiation of pluripotent human EC cells.