Thy-1 positive tingible body macrophages (TBM) in mouse lymph nodes.
Thy-1 positive tingible body macrophages (TBM) in mouse lymph nodes.
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小鼠淋巴结中 Thy-1 阳性可着色体巨噬细胞 (TBM)。
DOI：
10.1002/ar.1092220410
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Szakal,AK
中科院分区：
文献类型：
--
作者：
Smith,JP;Kosco,MH;Tew,JG;Szakal,AK
本研究是由观察到小鼠淋巴结（LN）中具有易染体巨噬细胞（TBM）细胞学特征的细胞似乎为Thy-1阳性而引起的。本研究的目的是确定这些大细胞是否为TBM，并最终证明其对Thy-1的反应性。使用抗Thy-1和巨噬细胞标志物（包括F4/80和Ia抗原）的单克隆抗体（MoAb）在光镜（LM）和电镜（EM）水平上研究细胞。在原位和体外LN制备物中，通过LM证实了TBM对Thy-1抗原特异性MoAb的免疫细胞化学反应性。此外，对这些生发中心细胞的原位超微结构检查表明，在LM水平观察到的Thy-1反应性与核糖体和内质网以及它们的质膜有关。同样，这些细胞表达Ia抗原和巨噬细胞特异性抗原F4/80的胞浆内和膜反应性。这表明反应性是由于Thy-1抗原的主动合成，而不是由于任何吞噬的Thy-1阳性细胞的反应性。根据其生发中心位置和形态学特征，这些Thy-1反应性巨噬细胞被鉴定为TBM。因此，生发中心TBM代表表达巨噬细胞和胸腺细胞标志物的成熟巨噬细胞的独特的、有力的吞噬亚群。
This study was prompted by the observation that cells in mouse lymph nodes (LN) with cytological characteristics of tingible body macrophages (TBM) appeared to be Thy‐1 positive. The objective of this study was to determine if these large cells were TBM and to conclusively demonstrate their reactivity for Thy‐1. The cells were studied using monoclonal antibodies (MoAb) against Thy‐1 and macrophage markers including F4/80 and Ia antigens at both light microscopic (LM) and electron microscopic (EM) levels. Immunocytochemical reactivity by TBM for Thy‐1 antigen specific MoAb was demonstrated by LM in both in situ and in vitro LN preparations. Furthermore, ultrastructural examination of these germinal center cells in situ demonstrated that the Thy‐1 reactivity visualized at the LM level was associated with ribosomes and endoplasmic reticulum as well as their plasma membrane. Similarly, these cells expressed intracytoplasmic and membrane reactivity for Ia antigens and also for the macrophage specific antigen F4/80. This indicates that the reactivity is due to active synthesis of the Thy‐1 antigen and not attributable to reactivity of any phagocytosed Thy‐1 positive cells. As defined by their germinal center location and morphological characteristics, these Thy‐1 reactive macrophages were identified as TBM. Germinal center TBM thus represent a unique, vigorously phagocytic subset of mature macrophages which express both macrophage and thymocyte markers.