Effect of age on alveolar bone turnover adjacent to maxillary molar roots in male rats: A histomorphometric study
Effect of age on alveolar bone turnover adjacent to maxillary molar roots in male rats: A histomorphometric study
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DOI：
10.1016/j.archoralbio.2006.06.012
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Sahara, Noriyuki
中科院分区：
文献类型：
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作者：
Misawa, Yasuko;Kageyama, Toru;Sahara, Noriyuki
目的：年龄对相邻的上颌磨牙根的雄性大鼠的牙槽骨营业额的影响进行了评估，特别注重骨形成和resorptation.Design：共110只雄性Wistar大鼠，年龄从6到100周，用于本研究。组织形态计量学参数进行了测量，在荧光标记的undecalcified地面和石蜡包埋脱钙部分的牙槽壁周围的远中舌根的上颌第一磨牙。通过测定双标记表面的骨表面百分比（dLS/BS）、骨形成率（BFR/BS）和矿物质沉积率（MAR）来静态测量骨形成。骨吸收进行了定量统计破骨细胞的数量（N.OC/BS）和破骨细胞（OC.S/BS）覆盖的骨表面的百分比。结果：对于牙槽壁的总表面，骨形成和吸收的所有参数的值随着年龄的增长而下降。所有这些值迅速下降，在生命的早期部分，从6至30-40周龄的大鼠。每个年龄组的牙槽骨壁的远中侧之间存在部位特异性差异。近中侧的dLS/BS和BFR/BS显著大于远中侧（p < 0.0001）。另一方面，远端的N.Oc/BS和Oc.S/BS值显著高于近中端（p < 0.0001）。而远中侧dLS/BS、BFR/BS和近中侧N.Oc/BS、Oc.S/BS在整个观察期内均无明显的增龄性变化。本研究的结果表明，随着年龄的增长，雄性大鼠的牙槽骨转换率迅速下降，但为了保持牙齿功能的完整性，机械应力可能仍然参与了骨形成，甚至在100周龄的大鼠中也存在肺泡壁的吸收。(c)2006爱思唯尔有限公司保留所有权利。
Objective: The effect of age on alveolar bone turnover adjacent to maxillary molar roots of male rats was assessed histomorphometrically with special focus on bone formation and resorption.Design: A total of 110 male Wistar rats ranging in age from 6 to 100 weeks were used for this study. Histomorphometric parameters were measured in fluorescence-labeled undecalcified ground and paraffin-embedded decalcified sections of the alveolar wall around the disto-lingual roots of the maxillary first molars. Bone formation was measured statically by determining the percentage of the bone surface that was double-labeled surface (dLS/BS), bone formation rate (BFR/BS), and mineral apposition rate (MAR). Bone resorption was quantified statically in terms of the number of osteoclasts (N.Oc/BS) and the percentage of the bone surface covered with osteoclasts (Oc.S/BS).Results: For the total surface of the alveolar wall, the values obtained for all parameters of both bone formation and resorption decreased with advancing age. All these values rapidly decreased during the early part of the life span, from 6 to 30-40 weeks of age, of the rats. A site-specific difference between the distal and mesial sides of the alveolar wall was found for each age group. dLS/BS and BFR/BS were significantly greater (p < 0.0001) on the mesial side than on the distal one. On the other hand, the distal side showed significantly greater (p < 0.0001) value for N.Oc/BS and Oc.S/BS did the mesial one. However, there were no significant age-related changes in dLS/BS and BFR/BS on the distal side or in N.Oc/BS and Oc.S/BS on the mesial side throughout observation period.Conclusion: The results of the present study demonstrate that alveolar bone turnover of male rats decreased rapidly with advancing age but that in order to maintain the integrity of the tooth function mechanical stress may still have participated in bone formation and resorption of the alveolar wall even in rats 100-week old. (c) 2006 Elsevier Ltd. All rights reserved.