The effect of body temperature on the murine electroretinogram
The effect of body temperature on the murine electroretinogram
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DOI：
10.1023/a:1022988332578
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发表时间：
2003-05-01
影响因子：
1.4
通讯作者：
Gouras, P
中科院分区：
文献类型：
--
作者：
Kong, H;Gouras, P
目的：研究体温对小鼠视网膜电图（ERG）的影响。研究方法：用生理盐水灯芯电极记录暗适应小鼠闪光灯引起的角膜ERG，同时连续测量直肠温度。将小鼠置于圆柱形盘管内，水从温控浴中循环通过该盘管。小鼠的体温在30至37 ℃之间逐步变化。结果：在体温30 ~ 37 ℃范围内，记录到的ERG基本上是正常形态。a波和b波的最大振幅随温度呈线性变化。b波振幅的变化率约为100 μ V/度。在30 ℃时，最大b波振幅约为400 μ V;在37 ℃时约为1000 μ V。体温的变化引起ERG振幅的快速变化。结论：小鼠视网膜电图对温度变化非常敏感。为了随着时间的推移准确地监测ERG，连续记录体温是必不可少的。
Purpose: To study the effect of body temperature on the murine electroretinogram (ERG). Methods: The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37degreesC. Results: ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37degreesC. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 muV/degree. At 30degreesC, maximum b-wave amplitude was about 400 muV; at 37degreesC it was about 1000 muV. A change in body temperature produced a rapid change in ERG amplitude. Conclusion: The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.