Nano-aggregate-Fe3+ complex based on benzimidazole-modified calix[4] arene for amplified fluorescence detection of ADP in aqueous media
Nano-aggregate-Fe3+ complex based on benzimidazole-modified calix[4] arene for amplified fluorescence detection of ADP in aqueous media
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DOI：
10.1016/j.snb.2018.12.116
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发表时间：
2019-04-01
影响因子：
8.4
通讯作者：
Jang, Doo Ok
中科院分区：
文献类型：
--
作者：
Kaur, Harpreet;Singh, Narinder;Jang, Doo Ok
在本研究中，合成了一种在杯[4]芳烃的下边缘1，3-交替位置（L1）附加的二足2-（2-氨基苯基）苯并咪唑支架。受体L1（O 1）的有机纳米颗粒在水溶液中通过关闭荧光响应对Fe 3+离子显示出显著的选择性和灵敏度。进一步研究了O 1中心点Fe 3+复合物对生物普遍阴离子的结合能力，并在纳摩尔范围内对ADP表现出优异的选择性，即使在结构相似的核苷酸存在下，荧光发射强度也有蓝移和四倍增强。ADP的测定是至关重要的，因为ADP在ATP酶和激酶催化的各种生物反应中起着重要作用。ATP和ADP也已知通过磷酸键裂解在细胞中产生能量。加入Fe 3+离子后的荧光猝灭可归因于纳米聚集体的聚集，当ADP加入到系综中时，纳米聚集体分离或进一步组装。因此，可以恢复系综的荧光。将该传感系统应用于血清样品中ADP的检测，回收率高达99.3%，验证了该传感系统的实用性。开关传感模式，简单的制造工艺，以及易于合成的方法是进一步应用的一些优势。该传感器还成功地应用于腺苷三磷酸双磷酸酶水解ADP为AMP的监测，这表明其在ADP相关生物活性的潜在应用。
In this study, a dipodal 2-(2-aminophenyl) benzimidazole scaffold appended to the lower rim of calix[4] arene at 1,3-alternate positions (L1) was synthesized. Organic nanoparticles of receptor L1 (O1) displayed remarkable selectivity and sensitivity for Fe3+ ions via a turn-off fluorescence response in an aqueous medium. The O1 center dot Fe3+ complex was further studied for its binding ability toward biologically prevalent anions, and showed excellent selectivity for ADP in the nanomolar range, even in the presence of structurally similar nucleotides, with a blue shift and four-fold enhancement in the intensity of fluorescence emission. The determination of ADP is crucial because ADP plays significant roles in various biological reactions catalyzed by ATPases and kinases. ATP and ADP are also known to generate energy in cells via phosphate bond cleavage. The fluorescence quenching upon addition of Fe3+ ions could be attributed to the conglomeration of the nano-aggregates, which either separated or further assembled when ADP was added to the ensemble. Consequently, the fluorescence of the ensemble could be recovered. The sensing system was also applied to the detection of ADP in spiked blood serum samples; the excellent recovery of up to 99.3% validated its practicality. The off-on sensing mode, simple fabrication process, and ease of synthetic methodology are some of the advantages for further applications. The sensor was also successfully applied to monitoring of the hydrolysis of ADP to AMP by apyrase, which indicated its potential application for ADP-pertinent biological activities.