SYNTHESIS OF MESSENGER-RNA GUANYLYLTRANSFERASE AND MESSENGER-RNA METHYLTRANSFERASES IN CELLS INFECTED WITH VACCINIA VIRUS
SYNTHESIS OF MESSENGER-RNA GUANYLYLTRANSFERASE AND MESSENGER-RNA METHYLTRANSFERASES IN CELLS INFECTED WITH VACCINIA VIRUS
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DOI：
10.1128/jvi.21.2.475-483.1977
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发表时间：
1977-01-01
影响因子：
5.4
通讯作者：
MOSS, B
中科院分区：
文献类型：
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作者：
BOONE, RF;ENSINGER, MJ;MOSS, B
修饰病毒mRNA的5“-末端以形成结构m7 G（5”）pppAm-和m7 G（5“）pppGm-的鸟苷酰转移酶和甲基转移酶似乎在牛痘病毒感染HeLa细胞后合成。在病毒接种后1小时内检测到这些酶活性的升高，并在4-10小时增加15 - 30倍。增加鸟苷酰和甲基转移酶的活动被阻止放线菌酮，蛋白质合成的抑制剂，但不是由胞嘧啶阿拉伯糖苷，DNA合成的抑制剂。mRNA鸟苷酰转移酶和甲基转移酶可能是早期或复制前的病毒基因产物。鸟苷酰转移酶和2种甲基转移酶，一种鸟嘌呤-7-甲基转移酶和一种核苷-2“-甲基转移酶，通过柱色谱法从感染的细胞提取物中分离出来，并且具有与先前从牛痘病毒核心中分离的相应酶相似或相同的性质。具有这些特性的酶不能从未感染的细胞中分离出来。
Guanylyltransferase and methyltransferases that modify the 5''-terminals of viral mRNA to form the structures m7G(5'')pppAm- and in m7G(5'')pppGm-appear to be synthesized after vaccinia virus infection of HeLa cells. Elevations in these enzyme activities were detected within 1 h after virus inoculation and increased 15 to 30-fold by 4-10 h. Increases in the guanylyl- and methyltransferase activities were prevented by cycloheximide, an inhibitor of protein synthesis, but not by cytosine arabinoside, an inhibitor of DNA synthesis. The mRNA guanylyl- and methyltransferases are probably early or prereplicative viral gene products. The guanylyltransferase and 2 methyltransferases, a guanine-7-methyltransferase and a nucleoside-2''-methyltransferase, were isolated by column chromatography from infected cell extracts and had properties similar or identical to those of the corresponding enzymes previously isolated from vaccinia virus cores. Enzymes with these properties could not be isolated from uninfected cells.