Therapeutic Role of Tangshenkang Granule in Rat Model with Diabetic Nephropathy
Therapeutic Role of Tangshenkang Granule in Rat Model with Diabetic Nephropathy
复制标题
糖肾康颗粒对糖尿病肾病大鼠模型的治疗作用
DOI：
10.1007/s11655-016-2607-x
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发表时间：
2018-08-01
影响因子：
2.9
通讯作者：
Wang Yong-jun
中科院分区：
文献类型：
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作者：
Hu Shun-jin;Shu Bing;Wang Yong-jun
目的：观察糖肾康颗粒对糖尿病肾病（DN）大鼠的肾脏保护作用。方法：40只雄性SD大鼠随机分为对照组、糖尿病肾病组、糖肾康组和苯那普利组。糖尿病肾病组、糖肾康组、苯那普利组建立糖尿病肾病模型。糖肾康组和苯那普利组分别每天灌胃糖肾康颗粒溶液和盐酸苯那普利溶液，连续8周。检测尿白蛋白和肌酐。除计算24 h尿蛋白（24 h UPr）、血清肌酐（Scr）、血尿素氮（BUN）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）和肌酐清除率（Ccr）外，还计算白蛋白/肌酐（ACR）。取右肾行高碘酸-次甲氨基银-Masson染色，观察病理变化。测量肾小球平均直径（DG）、肾小球平均直径（AG）和系膜区面积（AM）。透射电镜观察肾小球基底膜（TGBM）厚度。结果如下：与对照组相比，ON组大鼠体重明显减轻，肥大指数明显升高，24 h尿量、24 h UPr、ACR、Scr、BUN、Ccr、血脂及肾脏病理指标DG、AG、AM、AM/AG、TGBM明显升高（P
Objective: To evaluate the renal protective effect of Tangshenkang Granule in a rat model of diabetic nephropathy (DN). Methods: Forty male Sprague-Dawley rats were randomly divided into control, DN, Tangshenkang and benazepril groups. DN model was established in the rats of DN, Tangshenkang and benazepril groups. Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks, respectively. Urinary albumin and creatinine were detected. The albumin/creatinine (ACR) was calculated in addition to 24 h urinary protein (24-h UPr), serum creatinine (Scr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and creatinine clearance rate (Ccr). Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining. The average glomerular diameter (DG), average glomerular (AG) and mesangial areas (AM) were measured. The thickness of glomerular basement membrane (TGBM) was detected using transmission electron microscope. Results: Compared with rats in the control group, rats in the ON group showed significantly decreased body weight, increased hypertrophy index, 24-h urinary volume, 24-h UPr, ACR, Scr, BUN, Ccr, blood lipids as well as renal pathological indices including DG, AG, AM, AM/AG and TGBM (P