Identification of secreted CD155 isoforms
Identification of secreted CD155 isoforms
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DOI：
10.1016/s0006-291x(03)01560-2
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发表时间：
2003-09-12
影响因子：
3.1
通讯作者：
Denis, MG
中科院分区：
文献类型：
--
作者：
Baury, W;Masson, D;Denis, MG
CD155基因是免疫球蛋白超家族的成员。我们首先证明了可溶性CD 155（sCD 155）亚型的存在条件下的CD 155表达细胞的培养基中，在人血清和脑脊液中。使用特异性ELISA测量人血清和脑脊液中的sCD 155浓度。条件培养基的分析表明，sCD 155的释放不需要蛋白酶活性。为了确定哪些组织负责sCD 155表达，我们定量了人正常组织中的CD 155 mRNA。在肝脏中观察到最高表达。CD 155 α转录物是最丰富的，并且CD 155 β和CD 155 γ变体的比例在组织之间相似。最后，血清纯化的sCD 155可减少膜结合CD 155介导的脊髓灰质炎病毒进入。许多组织中CD 155的高水平合成和生物体液中sCD 155的存在表明该蛋白在细胞功能中具有重要作用。(C)2003年爱思唯尔公司All rights reserved.
The CD155 gene is a member of the immunoglobulin superfamily. We first demonstrate the existence of soluble CD155 (sCD155) isoforms in culture medium conditioned by CD155-expressing cells, in human serum and in cerebrospinal fluid. sCD155 concentration was measured in human serum and cerebrospinal fluid using a specific ELISA. Analysis of conditioned media indicated that sCD155 release does not require protease activity. In order to determine which tissues are responsible for sCD155 expression, we have quantified CD 155 mRNAs in human normal tissues. The highest expression was observed in liver. The CD 155alpha transcript is the most abundant and the proportion of the CD155beta and CD155gamma variants was similar between the tissues. Finally, serum purified sCD155 reduces poliovirus entry mediated by membrane-bound CD155. The high level of CD 155 synthesis in many tissues and the presence of sCD155 in biological fluids suggest the existence of an important role for the protein in cellular function. (C) 2003 Elsevier Inc. All rights reserved.