Comparative immunohistochemical evaluation of the zonal distribution of extracellular matrix and inflammation markers in human meniscus in osteoarthritis and rheumatoid arthritis
Comparative immunohistochemical evaluation of the zonal distribution of extracellular matrix and inflammation markers in human meniscus in osteoarthritis and rheumatoid arthritis
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DOI：
10.1016/j.acthis.2014.12.009
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Schumacher, Udo
中科院分区：
文献类型：
--
作者：
Fuhrmann, Inga Katharina;Steinhagen, Joern;Schumacher, Udo
本研究的目的是分析类风湿性关节炎和骨关节炎患者半月板中主要细胞外基质糖胺聚糖透明质酸(HA)及其受体CD44的分布，以及影响细胞外基质(T细胞和B细胞、巨噬细胞、内皮细胞)重建的细胞，以分析这些标志物是否有助于鉴别这两种关节疾病。半月板取自全膝关节置换术患者。组织学染色(H&E、PAS/Alcian Blue中性和带电碳水化合物残基)和免疫组织化学检测HA、CD44、1-磷酸鞘氨醇受体1(EDG-1)作为内皮细胞标志、CD3作为T细胞标志、CD20作为B细胞标志和CD68作为巨噬细胞标志。在两种疾病中，与半月板血管化较差的区域相比，血管化区域的细胞外基质显示出更多的HA和酸性碳水化合物残留量。EDG-1阳性的内皮细胞在所有区域均可见，类风湿半月板内区的细胞数比骨关节炎的少。巨噬细胞、T细胞、B细胞以及CD44阳性细胞在半月板血管化程度较低的中央区更为明显。滑膜周围区细胞外基质成分、CD44阳性细胞和炎症标志物的分布规律与滑膜组织相似，提示滑膜和半月板均参与了骨关节炎和类风湿关节炎的病理改变。结论：在两种关节疾病中，细胞外糖结合物和调节其合成的细胞的分布显示出相似的结果，不能区分类风湿性关节炎和骨性关节炎，但强调了这些标记物在人类半月板炎症和降解中的作用。(C)2015年爱思唯尔股份有限公司。版权所有。
The purpose of our study was to analyze the distribution of the major extracellular matrix glycosaminoglycan hyaluronan (HA), its receptor CD44 and cells which influence (re)modeling of the extracellular matrix (T- and B-cells, macrophages, endothelial cells) in menisci obtained from patients suffering from rheumatoid arthritis or osteoarthritis in order to analyze whether these markers could be useful to differentiate between both arthropathies. Human menisci were sampled from patients undergoing total knee arthroplasty. Histological staining (H&E, PAS/Alcian Blue for neutral and charged carbohydrate residues) and (immuno)histochemistry were performed for detection of HA, CD44, sphingosine-1-phosphate receptor 1 (EDG-1) as a marker for endothelial cells, CD3 as a marker for T-cells, CD20 as a marker for B-cells and CD68 as a marker for macrophages. The extracellular matrix in the vascularized zone showed higher amounts of HA as well as acid carbohydrate residues in comparison to the poorly vascularized zones of the meniscus in both disease entities. EDG-1 positive endothelial cells were present in all zones, with fewer cells being detected in the inner zones of the rheumatoid menisci than in the osteoarthritic ones. Macrophages, T- and B-cells as well as CD44-positive cells were more prominent in the vascularized zone of the meniscus than in the poorly vascularized central zone. The distribution patterns of the extracellular matrix components as well as the CD44-positive cells and the inflammation markers in the peripheral zone resembled the distribution in synovial tissue, indicating that both synovia and meniscus were involved in pathological changes in osteoarthritis and rheumatoid arthritis. In conclusion: the distribution of extracellular glycoconjugates and of cells modulating their synthesis showed similar results in both arthropathies, not enabling a differentiation between rheumatoid arthritis and osteoarthritis but underlining the role of these markers in inflammation and degradation in human meniscus. (C) 2015 Elsevier GmbH. All rights reserved.