Prevalence and Distribution of Salmonella in Organic and Conventional Broiler Poultry Farms
Prevalence and Distribution of Salmonella in Organic and Conventional Broiler Poultry Farms
复制标题
DOI：
10.1089/fpd.2010.0566
复制
发表时间：
2010-11-01
影响因子：
2.8
通讯作者：
Gebreyes, Wondwossen A.
中科院分区：
文献类型：
--
作者：
Alali, Walid Q.;Thakur, Siddhartha;Gebreyes, Wondwossen A.
本横断面研究的目的是比较沙门氏菌和抗微生物沙门氏菌的流行情况，并调查该病原体在有机和传统肉鸡养殖场的分布情况。从3周龄和8周龄的禽类中采集粪便（n = 420）、饲料（n = 140）和饮用水（n = 140）样本，进行2个鸟群周期。在北卡罗来纳州同一家公司的三个有机肉鸡农场和四个传统肉鸡农场，每个农场都有一个鸡舍的样本。使用选择性富集技术分析所有样品是否存在沙门氏菌。进一步进行表型（抗菌药物敏感性）和基因型（脉冲场凝胶电泳[PFGE]）检测。有机养殖场和常规养殖场粪便沙门氏菌检出率分别为5.6%（10/180）和38.8%（93/240）。饲料中，有机养殖场沙门氏菌检出率为5.0%(3/60)，传统养殖场为27.5%（22/80）。所有水样均未检出沙门氏菌。70株菌株具有抗菌敏感性和PFGE类型。最常见的两种耐药表型是对链霉素的单一耐药（36.2%[25/58]：常规耐药；25%[3/12]有机耐药）和对氨苄西林-链霉素-阿莫西林/克拉维酸-头孢菌素-头孢呋丁-头孢西丁6种抗菌药物的多重耐药（AmStAxChCfFx； 39.7%：常规耐药）。PFGE基因型分析显示两种养殖场内部和之间的分离株具有克隆性。我们的研究结果表明，在该家禽公司内，经过认证的有机家禽中粪便沙门氏菌的流行率低于常规饲养的家禽，并且抗微生物沙门氏菌的流行率也高于常规饲养的有机家禽。
The objective of this cross-sectional study was to compare the prevalence of Salmonella and antimicrobial-resistant Salmonella, as well as investigate the distribution of this pathogen in organic and conventional broiler poultry farms. Fecal (n = 420), feed (n = 140), and drinking water (n = 140) samples were collected from birds at 3 and 8 weeks of age for 2-flock cycles. One house was sampled per farm at three organic and four conventional broiler farms from the same company in North Carolina. All samples were analyzed for the presence of Salmonella using selective enrichment techniques. Further phenotypic (antimicrobial susceptibility) and genotypic (pulsed-field gel electrophoresis [PFGE]) testing were performed. Salmonella prevalences in fecal samples were 5.6% (10/180) and 38.8% (93/240) from organic and conventional farms, respectively. From feed, 5.0% (3/60) and 27.5% (22/80) of the samples were positive for Salmonella from organic and conventional farms, respectively. None of the water samples were positive for Salmonella. Seventy isolates were characterized by antimicrobial susceptibility and PFGE types. The two most common resistance phenotypes were single resistance to streptomycin (36.2% [25/58]: conventional; 25% [3/12] organic), and multidrug resistance to six antimicrobial agents: ampicillin-streptomycin-amoxicillin/clavulanic acid-cephalothin-ceftiofur-cefoxitin (AmStAxChCfFx; 39.7%: conventional only). Genotypic analysis using PFGE showed clonality among isolates within and between the two types of farms. The results of our study suggest that within this poultry company, the prevalence of fecal Salmonella was lower in certified-organic birds than in conventionally raised birds, and the prevalence of antimicrobial-resistant Salmonella was also higher in conventionally raised birds than in certified-organic birds.