FLUORESCENCE LIFETIME-BASED SENSING IN TISSUES - A COMPUTATIONAL STUDY
FLUORESCENCE LIFETIME-BASED SENSING IN TISSUES - A COMPUTATIONAL STUDY
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DOI：
10.1016/s0006-3495(95)80330-9
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发表时间：
1995-04-01
影响因子：
3.4
通讯作者：
SEVICKMURACA, EM
中科院分区：
文献类型：
--
作者：
HUTCHINSON, CL;LAKOWICZ, JR;SEVICKMURACA, EM
我们对光子扩散方程进行了数值求解，以预测具有均匀荧光团浓度的组织模型系统中的光分布。我们的结果表明，光传播的时间依赖性测量可用于监测组织中均匀分布的荧光团的荧光寿命。通过适当的参考，相移 θ 的频域测量可以实现荧光寿命 tau 的定量，而与局部吸收和散射特性的变化无关。这些结果表明，当荧光团的寿命与光子迁移时间相当时，通过定量荧光寿命 tau 来进行无创诊断监测的新方法。
We have numerically solved the photon diffusion equation to predict the distribution of light ina tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption nd scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.