Chloramphenicol-sensitive mitochondrial translation in Trypanosoma brucei
Chloramphenicol-sensitive mitochondrial translation in Trypanosoma brucei
复制标题
布氏锥虫中氯霉素敏感的线粒体翻译
作者：
C. Nabholz;D. Speijer;A. Schneider
Abstract We developed an in organello system to label newly synthesized mitochondrially encoded proteins of Trypanosoma brucei. Highly purified mitochondria, prepared under isotonic conditions, were incubated with radioactive methionine and cysteine in a suitable translation buffer. Analysis of mitochondrial extracts on TRIS-Tricine gels revealed a subset of labeled, NP-40-insoluble proteins. The labeling of these proteins was resistant to the cytosol-specific translation inhibitor cycloheximide. The proteins, however, were not labeled in the presence of chloramphenicol or erythromycin, inhibitors of prokaryotic type translation, or puromycin, a general translation inhibitor. These results indicate that isotonically isolated mitochondria of T. brucei are capable of protein synthesis.
氯霉素异构体和其他抗生素对前环布氏锥虫的蛋白质合成和呼吸的抑制作用。
影响因子：
1.5
作者：
Spithill,TW;Shimer,SP;Hill,GC
通讯作者：
Hill,GC
在锥虫线粒体中，尿苷添加到编辑过的 RNA 中的发生与转录无关。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Harris,ME;Moore,DR;Hajduk,SL
通讯作者：
Hajduk,SL
细长血流中存在鱼藤酮敏感的 NADH 脱氢酶和布氏锥虫的前循环形式。
DOI：
10.1111/j.1432-1033.1996.00888.x
发表时间：
1996
期刊：
European journal of biochemistry
影响因子：
--
作者：
Beattie,DS;Howton,MM
通讯作者：
Howton,MM