Recovery and screening for antibiotic susceptibility of potential bacterial pathogens from the oral cavity of shark species involved in attacks on humans in Recife, Brazil
Recovery and screening for antibiotic susceptibility of potential bacterial pathogens from the oral cavity of shark species involved in attacks on humans in Recife, Brazil
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DOI：
10.1099/jmm.0.020453-0
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发表时间：
2010-08-01
影响因子：
3
通讯作者：
Lima-Filho, J. V.
中科院分区：
文献类型：
--
作者：
Interaminense, J. A.;Nascimento, D. C. O.;Lima-Filho, J. V.
在巴西东北部海岸累西腓的海滩上，鲨鱼和人类发生的事件数量是世界上最多的。此外，幸存者的伤口感染很常见；但病因的性质和风险尚不清楚。本研究在累西腓参与袭击的鲨鱼口腔中鉴定出81种潜在的细菌病原体，并采用标准化圆盘扩散法进行抗生素药敏试验。其中以肠杆菌、柠檬酸杆菌、变形杆菌、碱性普罗维登斯菌、大肠杆菌、威斯康星莫勒菌和棕榈乳杆菌等肠杆菌为主。革兰氏阴性菌包括弧菌、洋葱伯克霍氏菌、不动杆菌和假单胞菌，除绿绿菌组的链球菌外，还检出凝固酶阳性和凝固酶阴性的葡萄球菌、肠球菌和微球菌。在变形杆菌和弗氏柠檬酸杆菌中尤其发现了耐药性，在测试的13种抗生素中，耐药性从4到6种不等。庆大霉素和万古霉素对革兰氏阳性球菌菌株最有效，而左氧氟沙星对革兰氏阳性和革兰氏阴性细菌均有完全抑制作用。这些数据是根据对在累西腓Restauracao医院治疗的三名鲨鱼受害者的医疗记录的回顾性评估来讨论的。
The number of incidents involving sharks and humans at beaches in Recife, on the north-eastern Brazilian coast, is among the highest worldwide. In addition, wound infections in survivors are common; but the nature and risk of the aetiological agents is unknown. In the present study, 81 potential bacterial pathogens were identified in the oral cavity of sharks involved in attacks in Recife, and were subjected to antibiotic susceptibility tests using the standardized disc-diffusion method. The majority were enterobacteria such as Enterobacter spp., Citrobacter spp., Proteus spp., Providencia alcalifaciens, Escherichia coli, Moellerella wisconcensis and Leclercia adecarboxylata. Other Gram-negative bacteria included Vibrio spp., Burkholderia cepacia, Acinetobacter spp. and Pseudomonas spp. In addition, coagulase-positive and coagulase-negative Staphylococcus spp., Enterococcus spp. and Micrococcus spp. were identified, besides Streptococcus spp. from the viridans group. Resistance was especially found in the Proteus mirabilis and Citrobacter freundii, and ranged from 4 to 6 antibiotics out of the 13 tested. Gentamicin and vancomycin were the most effective against Gram-positive cocci strains, whereas levofloxacin was fully inhibitory against Gram-positive and Gram-negative bacteria. These data are discussed in light of a retrospective evaluation of the medical records of three shark victims treated at Restauracao Hospital in Recife.