Discovery of novel photosensitized nanoparticles as a preservative for the storage of strawberries and their activity against Botrytis cinerea
Discovery of novel photosensitized nanoparticles as a preservative for the storage of strawberries and their activity against Botrytis cinerea
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DOI：
10.1016/j.lwt.2021.111359
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发表时间：
2021-03
期刊：
影响因子：
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通讯作者：
X. Niu;Lu Liu;Hongsu Wang;Li Lin;Yanan Yang;Yawen Gao;Xiyan Wang;Jiao Sun;Biao Dong
中科院分区：
文献类型：
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作者：
X. Niu;Lu Liu;Hongsu Wang;Li Lin;Yanan Yang;Yawen Gao;Xiyan Wang;Jiao Sun;Biao Dong
本文报道了一种基于光动力疗法（PDT）的新型抗真菌纳米粒子Fe3O4@Ce6/C6@silane（NPs），该纳米粒子具有良好的抗灰葡萄孢活性。由于Fe_3 O_4@Ce_6/C_6@silane具有显著的光敏化特性，在红光照射下光敏剂Ce_6产生单线态氧（1 O_2），导致Fe_3 O_4@Ce_6/C_6@silane对番茄灰霉病菌具有抑制活性。Fe3O4@Ce6/C6@silane对灰葡萄孢孢子萌发和菌丝生长的半最大抑制浓度（IC 50）分别为0.022611和0.014213 g L−1。Ce 6产生的1 O2被证实是实际的抗真菌活性成分，而不是辐射本身。此外，通过构建感染灰葡萄孢的草莓的感染模型，发现灰葡萄孢在空白对照组和阴性对照组的草莓中正常生长，而在红光照射下用NP处理的草莓在保存的8d内没有抑制灰葡萄孢的生长。结果表明，Fe3O4@Ce6/C6@silane对番茄灰霉病菌有很好的抑制作用，其抑制作用是通过Ce 6释放1 O2实现的，可作为一种潜在的水果光敏保鲜剂。
In this work, a novel antifungal Nanoparticles (NPs), Fe3O4@Ce6/C6@silane, based on photodynamic therapy (PDT) with excellent anti-Botrytis cinereaactivity was reported. Due to the remarkable photosensitization properties of Fe3O4@Ce6/C6@silane, singlet oxygen (1O2) was generated from the photosensitizer Ce6 under red light irradiation, leading to the inhibitory activity of Fe3O4@Ce6/C6@silane againstBotrytis cinerea. The half-maximal inhibitory concentrations (IC50) of Fe3O4@Ce6/C6@silane towards spore germination and hyphal growth inBotrytis cinereawere 0.022611 and 0.014213 g L−1, respectively.1O2generated from Ce6 was confirmed to be the actual antifungal active ingredient, not the irradiation itself. Furthermore, via the infection model involving construction of strawberries infected withBotrytis cinerea, it was found thatBotrytis cinereagrew normally in strawberries from the blank and negative control groups, whereas strawberries treated with NPs under red light irradiation did not exhibitBotrytis cinereagrowth within 8d of preservation. These results showed that Fe3O4@Ce6/C6@silane has an excellent inhibitory effect onBotrytis cinereabased on the release of1O2from Ce6 and could be used as a potential photosensitized preserving agent for fruit storage.