ATOMIC FORCE MICROSCOPY AND DISSECTION OF GAP-JUNCTIONS
ATOMIC FORCE MICROSCOPY AND DISSECTION OF GAP-JUNCTIONS
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DOI：
10.1126/science.1910206
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发表时间：
1991-09-20
期刊：
影响因子：
56.9
通讯作者：
ARNSDORF, MF
中科院分区：
文献类型：
--
作者：
HOH, JH;LAL, R;ARNSDORF, MF
原子力显微镜（AFM）被用于研究在磷酸盐缓冲盐水（PBS）中分离出的肝间隙连接的结构。这些间隙连接的厚度似乎是14.4纳米，接近电子显微镜（EM）所报道的尺寸。当对显微镜尖端施加的力逐渐增大时，间隙连接的上膜可以被“解剖”开，使下膜的细胞外结构域暴露出来。当对经胰蛋白酶处理且用戊二醛固定的样品进行这种“力解剖”时，间隙连接半通道的六边形阵列就会显现出来，其中心到中心的间距为9.1纳米。
An atomic force microscope (AFM) was used to study the structure of isolated hepatic gap junctions in phosphate-buffered saline (PBS). The thickness of these gap junctions appears to be 14.4 nanometers, close to the dimensions reported by electron microscopy (EM). When an increasing force is applied to the microscope tip, the top membrane of the gap junction can bc "dissected" away, leaving the extracellular domains of the bottom membrane exposed. When such "force dissection" is performed on samples both trypsinized and fixed with glutaraldehyde, the hexagonal array of gap junction hemichannels is revealed, with a center-to-center spacing of 9.1 nanometers.