Desulfovibrio confers resilience to the comorbidity of pain and anxiety in a mouse model of chronic inflammatory pain
Desulfovibrio confers resilience to the comorbidity of pain and anxiety in a mouse model of chronic inflammatory pain
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DOI：
10.1007/s00213-022-06277-4
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发表时间：
2022-11
影响因子：
3.4
通讯作者：
Zifeng Wu;Hanyu Liu;Enshi Yan;Xinying Zhang;Yuanyuan Wang;Chao Huang;Teng He;Liying Miao
中科院分区：
文献类型：
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作者：
Zifeng Wu;Hanyu Liu;Enshi Yan;Xinying Zhang;Yuanyuan Wang;Chao Huang;Teng He;Liying Miao
背景慢性疼痛患者常伴有焦虑症状。已经确定肠道微生物群与疼痛和焦虑的发病机制有关。然而，它是未知的肠道微生物群，特别是特定的细菌，发挥作用的慢性疼痛和anxiety.MethodsChronic炎性疼痛的并发症在小鼠中诱导由一个单一的注射完全弗氏佐剂（CFA）。然后通过行为的层次聚类分析将小鼠分为焦虑敏感和焦虑恢复表型。采集粪便样本进行16S rRNA基因测序。结果慢性地西泮干预对肠道菌群的α多样性和β多样性均有显著性差异。共有12种肠道细菌在CFA注射后发生改变，并通过慢性地西泮治疗逆转。更重要的是，慢性地西泮治疗缓解了疼痛超敏反应和焦虑样行为。有趣的是，我们还发现，脱硫弧菌是增加焦虑弹性组相比，控制和焦虑易感groups.ConclusionAbnormal肠道菌群的组成在慢性疼痛以及焦虑中起着至关重要的作用。此外，抗焦虑小鼠中脱硫弧菌水平的升高表明其对疼痛和焦虑的共病具有治疗作用。总的来说，靶向肠道微生物群，特别是增加脱硫弧菌水平，可能有效缓解慢性疼痛焦虑合并症。
BackgroundPatients with chronic pain frequently suffer from anxiety symptoms. It has been well established that gut microbiota is associated with the pathogenesis of pain and anxiety. However, it is unknown whether the gut microbiota, particularly the specific bacteria, play a role in the comorbidity of chronic pain and anxiety.MethodsChronic inflammatory pain was induced in mice by a single injection of complete Freund’s adjuvant (CFA). Mice were then separated into anxiety-susceptible and anxiety-resilient phenotypes by hierarchical clustering analysis of behaviors. Fecal samples were collected to perform 16S rRNA gene sequencing. Chronic diazepam intervention served as a therapeutic strategy and its effect on the composition of gut microbiota was also determined.Resultsα-Diversity and β-diversity both showed significant differences among the groups. A total of 12 gut bacteria were both altered after CFA injection and reversed by chronic diazepam treatment. More importantly, the pain hypersensitivity and anxiety-like behaviors were relieved by chronic diazepam treatment. Interestingly, we also found thatDesulfovibriowas increased in anxiety-resilient group compared to control and anxiety-susceptible groups.ConclusionAbnormal composition of gut microbiota plays an essential role in chronic pain as well as in anxiety. Besides, the increased level ofDesulfovibrioin anxiety-resilient mice indicated its therapeutic effects on the comorbidity of pain and anxiety. Collectively, targeting gut microbiota, especially increasing theDesulfovibriolevel, might be effective in the alleviation of chronic pain-anxiety comorbidity.