Molecular cloning of a novel melanocortin receptor.
Molecular cloning of a novel melanocortin receptor.
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DOI：
10.1016/s0021-9258(18)53088-x
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发表时间：
1993-04
期刊：
影响因子：
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通讯作者：
I. Gantz;Y. Konda;T. Tashiro;Y. Shimoto;Hiroto Miwa;G. Munzert;S. Watson;J. Delvalle;Thoru Yamada
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文献类型：
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作者：
I. Gantz;Y. Konda;T. Tashiro;Y. Shimoto;Hiroto Miwa;G. Munzert;S. Watson;J. Delvalle;Thoru Yamada
使用聚合酶链反应技术的基础上的同源跨膜区的7个跨膜G蛋白连接的受体的寡核苷酸引物，我们分离出三个全长的人类基因，编码一个新的亚组的这个受体家族。最近，这些受体中的两个被鉴定为特异性的α-黑素细胞刺激激素（α-MSH）和促肾上腺皮质激素。我们报告的分子克隆和药理学特性的第三个成员，这个小组。该受体的基因编码一种长度为361个氨基酸的蛋白质。其药理学特征是作为促肾上腺皮质激素和α-、β-和γ-MSH共有的七肽核心特异性的MSH受体。通过北方印迹杂交和聚合酶链反应，它在脑、胎盘和肠组织中表达，但在黑色素瘤细胞或肾上腺中不表达。这些发现可能会产生洞察的生理肽来自前阿黑皮素翻译后加工。
Using the technique of the polymerase chain reaction primed with oligonucleotides based on the homologous transmembrane regions of seven transmembrane G protein-linked receptors, we isolated three full-length human genes that encode a novel subgroup of this receptor family. Recently, two of these receptors were identified as specific for alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropic hormone. We report the molecular cloning and pharmacologic characterization of a third member of this subgroup. The gene for this receptor encodes a protein of 361 amino acids in length. Its pharmacology characterizes it as an MSH receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. By Northern blot hybridization and polymerase chain reaction, it is expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. These findings may yield insight into the physiology of peptides derived from pro-opiomelanocortin post-translational processing.