Agar/collagen membrane as skin dressing for wounds
Agar/collagen membrane as skin dressing for wounds
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DOI：
10.1088/1748-6041/3/4/044108
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发表时间：
2008-12-01
影响因子：
4
通讯作者：
Tang, Shunqing
中科院分区：
文献类型：
--
作者：
Bao, Lei;Yang, Wei;Tang, Shunqing
琼脂是一种高度亲水的聚合物，具有特殊的凝胶性质和良好的生物相容性，但在水环境下强度适中，降解率低。为了使ⅰ型胶原蛋白的力学强度和降解性能都符合要求，采用50℃干燥或冻干的方法，将ⅰ型胶原蛋白与琼脂按一定比例复合。选择戊二醛作为交联剂，最有利的交联条件是琼脂与戊二醛的质量比为66.7，pH值约为5。动态力学分析结果表明，单个琼脂膜的模量值在640 ~ 1064 MPa之间，而与I型胶原复合后的模量值在340 ~ 819 MPa之间。在光学显微镜下发现，复合支架中的孔隙是相互连接的，而不是单一I型胶原支架中的蜂窝状孔隙或单一琼脂支架中的层状间隙。急性毒性试验结果表明，复合材料虽然与一定浓度的戊二醛交联，但对小鼠没有毒性。大体检查结果表明，复合膜或支架用于修复兔皮肤病变时，复合膜或支架修复效果良好，覆盖的病变处无感染、无液体渗出、无明显瘢痕。而对照组自体皮肤出现坏死，病变部位出现大量瘢痕组织。H&E染色结果显示修复组织与正常组织相似，2 - 3周后几乎没有支架或膜未降解。综上所述，I型胶原蛋白增加了琼脂膜的韧性，琼脂/ I型胶原蛋白复合材料是一种很有前途的生物材料，可作为烧伤或溃疡愈合的伤口敷料。
Agar, a highly hydrophilic polymer, has a special gel property and favorable biocompatibility, but moderate intension strength in an aqueous condition and a low degradation rate. In order to tailor both properties of mechanical intension and degradation, type I collagen was composited with agar in a certain ratio by drying at 50 degrees C or by a freeze-dry process. Glutaraldehyde was chosen as a crosslinking agent, and the most favorable condition for crosslinking was that the weight ratio of agar to glutaraldehyde was 66.7 and the pH value about 5. Dynamic mechanical analysis results showed that the single agar membrane had a modulus value between 640 MPa and 1064 MPa, but it was between 340 MPa and 819 MPa after being composited with type I collagen. It was discovered under an optical microscope that the pores were interconnected in the composite scaffolds instead of the honeycomb-like pores in a single type I collagen scaffold or the laminated gaps in a single agar scaffold. The results of an acute toxicity test disclosed that the composites were not toxic to mice although the composites were crosslinked with a certain concentration of glutaraldehyde. The results of gross examinations showed that when the composite membranes or scaffolds were applied to repair a rabbit skin lesion, the composites had a good repair effect without infection, liquid exudation or visible scar in the lesion covered with them. But in the control group, the autologous skin showed necrosis and there were a lot of scar tissues in the lesion site. H&E staining results showed that the repair tissue was similar to the normal one and very few scaffolds or membranes were left without degradation after 2 or 3 weeks. In conclusion, it is proved that type I collagen increases the toughness of the agar membrane, and the agar/type I collagen composites are promising biomaterials as wound dressings for healing burns or ulcers.