N-acetoxy-acetylaminofluorene reacts preferentially with a control region of intracellular SV40 chromosome
N-acetoxy-acetylaminofluorene reacts preferentially with a control region of intracellular SV40 chromosome
复制标题
N-乙酰氧基-乙酰氨基芴优先与细胞内 SV40 染色体的控制区反应
作者：
P. Beard;M. Kaneko;P. Cerutti
许多化学致癌物或其代谢物与DNA1，2反应；因此，确定染色体结构对这些反应有什么影响是很有意义的。猴病毒40(SV40)的染色体非常适合于这样的研究；就像真核细胞的染色质一样，它被组织成核小体。SV40的核苷酸序列已知3，4，以及病毒基因表达和DNA复制的模式5，以及病毒染色体6-14的结构。我们研究了最终致癌物N-乙酰氧基乙酰氨基荧烯(AAAF)与完整感染细胞中SV40染色体特定区域的原位结合。这里报道的结果表明，细胞内SV40染色体上包含调控功能的区域具有独特的结构特性，使其比SV40基因组的其余部分更容易受到AAAF的攻击。AAAF与染色质调节区的优先结合可能对该致癌物和类似致癌物的作用机制有影响。
Many chemical carcinogens or their metabolites react with DNA1,2; thus it is of interest to determine what effect chromosomal structure has on these reactions. The chromosome of simian virus 40 (SV40) is well suited for such studies; like chromatin of eukaryotic cells, it is organized into nucleosomes. The nucleotide sequence of SV40 is known3,4, together with much about the pattern of viral gene expression and DNA replication5, and the structure of the viral chromosome6–14. We have investigated the binding of the ultimate carcinogen, N-acetoxy-acetylaminofluorene (AAAF), to specific regions of the SV40 chromosome in situ in the intact infected cell. The results, reported here, indicate that a region containing regulatory functions on the intracellular SV40 chromosome has unique structural properties which render it more susceptible to attack by AAAF than the rest of the SV40 genome. The preferential binding of AAAF to regulatory regions of chromatin may have implications for the mechanism of action of this and similar carcinogens.
HeLa 全细胞提取物中猿猴病毒 40 DNA 的转录。
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Handa,H;Kaufman,RJ;Manley,J;Gefter,M;Sharp,PA
通讯作者：
Sharp,PA