Propofol attenuates ovalbumin-induced smooth muscle contraction of the sensitized rat trachea: Inhibition of serotonergic and cholinergic signaling
Propofol attenuates ovalbumin-induced smooth muscle contraction of the sensitized rat trachea: Inhibition of serotonergic and cholinergic signaling
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DOI：
10.1213/01.ane.0000229853.01875.60
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发表时间：
2006-09-01
影响因子：
5.7
通讯作者：
Sumikawa, Koji
中科院分区：
文献类型：
--
作者：
Yamaguchi, Masakazu;Shibata, Osamu;Sumikawa, Koji
异丙酚被认为适用于支气管哮喘患者的麻醉诱导。然而，其作用于支气管的机制尚未完全了解。我们研究了异丙酚对卵清蛋白（OA）致敏大鼠气管收缩的影响。雄性Wistar大鼠通过单次腹腔注射OA 10 μ g与氢氧化铝10 mg混合作为佐剂致敏。14天后，使用气管环进行实验。我们观察了酮色林，一种5-HT 2受体拮抗剂，和阿托品对OA引起的收缩的影响。其次，观察异丙酚对OA-、5-羟色胺（5-HT）-、乙酰胆碱-或电场刺激诱导的收缩的影响。酮色林和阿托品联合使用可使OA诱导的收缩减弱90%。异丙酚以剂量依赖性方式显著减弱OA引起的收缩。异丙酚可消除5-HT引起的收缩，减弱乙酰胆碱引起的收缩，也几乎完全减弱5-HT对电场刺激引起的收缩的增强作用。这些结果表明，异丙酚减弱OA引起的收缩的机制是抑制5-HT的作用。异丙酚对免疫球蛋白E相关性哮喘患者是一种有用的麻醉剂。
Propofol is considered suitable for induction of anesthesia in patients with bronchial asthma. However, the mechanisms of its action on bronchi are not fully understood. We examined the effects of propofol on ovalbumin (OA)-induced contraction of OA-sensitized rat trachea. Male Wistar rats were sensitized by a single intraperitoneal injection of OA 10 mu g mixed with aluminum hydroxide, 10 mg, as adjuvant. Fourteen days later, the experiment was performed using the tracheal rings. We observed the effects of ketanserin, a 5-HT2 receptor antagonist, and atropine on OA-induced contraction. Next, the effects of propofol on OA-, serotonin (5-HT)-, acetylcholine-, or electrical field stimulation-induced contractions were observed. OA-induced contraction was 90% attenuated by the combination of ketanserin and atropine. Propofol significantly attenuated OA-induced contraction in a dose-dependent manner. Propofol abolished 5-HT-induced contraction, attenuated acetylcholine-induced contraction, and also almost completely attenuated the enhancement by 5-HT of electrical field stimulation-induced contraction. These results suggest that the mechanism involved in the attenuation by propofol of OA-induced contraction is inhibition of the actions of 5-HT. Propofol should be a useful anesthetic in patients with immunoglobulin E-related asthma.