Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157.
Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157.
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使用亚碲酸盐选择产细胞毒性大肠杆菌 O157。
DOI：
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发表时间：
1993
影响因子：
3
通讯作者：
C. A. Siddons
中科院分区：
文献类型：
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作者：
P. Zadik;P. Chapman;C. A. Siddons
亚碲酸钾的选择verocytogenic（VT+）大肠杆菌O 157进行了评估。VT+ E的MIC较高。coliO 157的活性高于其它菌株。大肠杆菌和气单胞菌属（Aeromonas spp.）在含有山梨醇、亚碲酸盐和头孢克肟的麦康凯培养基（TC-SMAC）上，VT+ E生长良好。对大肠杆菌O 157和宋内志贺菌的生长有抑制作用，而对其它大肠杆菌的生长有部分或完全抑制作用，抑制率为67%。大肠杆菌和所有或大多数其他受试的非发酵性种属的菌株。在TC-SMAC培养基上筛选的391份牛直肠拭子中，26份产生VT+ E分离株。coliO 157，而在含有头孢克肟和鼠李糖的M-M培养基中仅得到9株分离株。亚碲酸钾加入到麦康凯琼脂中可显著提高VT+ E的分离率。大肠杆菌O 157，也可用于其他类型的标本。
Potassium tellurite was assessed for the selection of verocytotoxigenic (VT+) Escherichia coli O157. MICs were higher for VT+ E. coli O157 than for other strains of E. coli and for Aeromonas spp. MacConkey medium containing sorbitol, tellurite and cefixime (TC-SMAC) permitted the growth of VT+ E. coli O157 and Shigella sonnei but partially or completely inhibited the growth of 67% of other strains of E. coli and all or most strains of other sorbitol-non-fermenting species tested. Of 391 rectal swabs from cattle screened on TC-SMAC medium, 26 yielded isolates of VT+ E. coli O157 whereas sorbitol-MacConkey medium with cefixime and rhamnose yielded only nine isolates. Inclusion of potassium tellurite in sorbitol-MacConkey agar markedly increased the rate of isolation of VT+ E. coli O157 from cattle rectal swabs and may do so for other types of specimen.