A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and structure.
A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and structure.
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DOI：
10.4049/jimmunol.127.2.596
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发表时间：
1981-08
影响因子：
4.4
通讯作者：
Konrad Kurzinger;Timothy Reynolds;Ronald N. Germain;D. Davignon;Eric Martz;T. A. Springer
中科院分区：
文献类型：
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作者：
Konrad Kurzinger;Timothy Reynolds;Ronald N. Germain;D. Davignon;Eric Martz;T. A. Springer
我们之前描述过一种单克隆抗体 (MAb) M7/14，它可以阻断多种 T 细胞功能，包括 CTL 介导的杀伤、混合淋巴细胞反应和抗原特异性增殖。 M7/14 定义的抗原被指定为淋巴细胞功能相关抗原一 (LFA-1)。在本报告中，对 LFA-1 的细胞分布、表面丰度、结构进行了研究，与其他 CTL 表面抗原相比，LFA-1 在 T 和 B 谱系的淋巴细胞以及大部分骨髓细胞上表达，但在渗出巨噬细胞或非淋巴组织上不表达。无论是在未刺激状态还是在刺激状态下，T 细胞都比 B 细胞表达更多的 LFA-1。与未刺激的脾细胞相比，溶细胞性 T 淋巴细胞制剂 (CTLP) 和 Con A 母细胞（但不是 LPS 母细胞）显示 LFA-1 表达相对于 H-2 和含有 T 细胞的群体 Lyt-2 有所增加。 M7/14 MAb 平均每个脾细胞或 CTLP 细胞分别结合约 1.5 X 10(4) 和 7 X 10(4) 个 LFA-1 位点。 M7/14 Mab 与 cTLP 的结合量分别比 H-2 和 Thy-1 Mab 少 2.5 倍和 10.4 倍；由于后者对 CTL 功能影响很小或没有影响，M7/14 MAb 的杀伤抑制作用是针对 LFA-1 表面位点的。 M7/14 MAb 和阻断性 Lyt-2 MAb 结合在相似数量的 CTLP 中。 LFA-1 是一种糖蛋白，由 180,000 和 95,000 Mr 的 2 条非共价连接的多肽链组成。与 CTL 相同的分子种类也存在于其他 T 细胞和 B 细胞上。 LFA-1 的分子结构和细胞分布清楚地将其与 Lyt-2,3、Ly-5、T145 和 T11 区分开来，后者先前被认为与 CTL 的功能相关和/或存在于 CTL 表面。
We have previously described a monoclonal antibody (MAb), M7/14, which blocks a variety of T cell functions, including CTL-mediated killing, the mixed lymphocyte response, and antigen-specific proliferation. The antigen defined by M7/14 has been designated lymphocyte function-associated antigen one (LFA-1). In this report, LFA-1 has been studied as to cell distribution, surface abundance, structure, and in comparison to other CTL surface antigens, LFA-1 is expressed on lymphoid cells of both the T and the B lineages and on a large fraction of bone marrow cells, but not on exudate macrophages or nonlymphoid tissues. T cells express more LFA-1 than B cells, both in the unstimulated and stimulated states. Compared with unstimulated spleen cells, cytolytic T lymphocyte cell preparations (CTLP) and Con A blasts, but not LPS blasts, show increased LFA-1 expression relative to H-2, and for T cell-containing populations, Lyt-2. M7/14 MAb binds to about 1.5 X 10(4) and 7 X 10(4) LFA-1 sites per average spleen cell or CTLP cell, respectively. M7/14 Mab binds to cTLP in quantitites of 2.5-fold and ies 10.4-fold less than H-2 and Thy-1 Mab, respectively; since the latter have little or no effect on CTL function, inhibition of killing by M7/14 MAb is specific for the LFA-1 surface site. M7/14 MAb and a blocking Lyt-2 MAb are bound in similar quantities of CTLP. LFA-1 is a glycoprotein and consists of 2 noncovalently linked polypeptide chains of 180,000 and 95,000 Mr. The same molecular species as on CTL is present on other T cells and on B cells. The molecular structure and cell distribution of LFA-1 clearly distinguishes it from Lyt-2,3, Ly-5, T145, and T11, which were previously suggested to be either associated with the function of and/or present on the surface of CTL.