Culturing Primary Human Osteoblasts on Electrospun Poly(lactic-co-glycolic acid) and Poly(lactic-co-glycolic acid)/Nanohydroxyapatite Scaffolds for Bone Tissue Engineering
Culturing Primary Human Osteoblasts on Electrospun Poly(lactic-co-glycolic acid) and Poly(lactic-co-glycolic acid)/Nanohydroxyapatite Scaffolds for Bone Tissue Engineering
复制标题
在用于骨组织工程的电纺聚（乳酸-乙醇酸）和聚（乳酸-乙醇酸）/纳米羟基磷灰石支架上培养原代人成骨细胞
DOI：
10.1021/am401937m
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发表时间：
2013-07-15
影响因子：
9.5
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Li, Mengmeng;Liu, Wenwen;Jiang, Xingyu
本研究以原代人成骨细胞为模型细胞，制备了骨组织工程用高分子纤维支架。通过采用一种简单的方法，静电纺丝，我们生产了不同形貌的聚（乳酸-羟基乙酸）（PLGA）支架，包括微球，珠状纤维，均匀的纤维，以及PLGA/纳米羟基磷灰石（纳米HA）复合支架。采用模拟体液（SBF）溶液研究了静电纺丝支架的骨结合能力，发现PLGA/nano-HA复合支架中的nano-HA能显著促进骨样磷灰石的形成。此外，我们利用小鼠前成骨细胞系（MC 3 T3 E1）和HOB进行了体外实验，以测试静电纺丝支架的性能。结果表明，PLGA/nano-HA纤维能有效促进HOB的增殖，是一种很有前景的骨修复支架材料。
In this work, we fabricated polymeric fibrous scaffolds for bone tissue engineering using primary human osteoblasts (HOB) as the model cell. By employing one simple approach, electrospinning, we produced poly(lactic-co-glycolic acid) (PLGA) scaffolds with different topographies including microspheres, beaded fibers, and uniform fibers, as well as the PLGA/nanohydroxyapatite (nano-HA) composite scaffold. The bone-bonding ability of electrospun scaffolds was investigated by using simulated body fluid (SBF) solution, and the nano-HA in PLGA/nano-HA composite scaffold can significantly enhance the formation of the bonelike apatites. Furthermore, we carried out in vitro experiments to test the performance of electrospun scaffolds by utilizing both mouse preosteoblast cell line (MC 3T3 E1) and HOB. Results including cell viability, alkaline phosphatase (ALP) activity, and osteocalcin concentration demonstrated that the PLGA/nano-HA fibers can promote the proliferation of HOB efficiently, indicating that it is a promising scaffold for human bone repair.