LncRNA TDRG1 promotes the aggressiveness of gastric carcinoma through regulating miR-873-5p/HDGF axis
LncRNA TDRG1 promotes the aggressiveness of gastric carcinoma through regulating miR-873-5p/HDGF axis
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DOI：
10.1016/j.biopha.2019.109425
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发表时间：
2020-01-01
影响因子：
7.5
通讯作者：
Li, Zhi Guo
中科院分区：
文献类型：
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作者：
Ma, Yan;Xu, Xiu Lian;Li, Zhi Guo
胃癌是最常见的消化系统肿瘤之一，也是恶性肿瘤死亡的主要原因。据报道，长链非编码RNA（lncRNA）在GC进展中起关键作用。在这项研究中，我们证明了lncRNA睾丸发育相关基因1（TDRG 1）在临床GC组织和GC细胞中显着上调。lncRNATDRG 1高表达与胃癌的转移和预后有关。在体外和体内，lncRNA TDRG 1的过表达促进GC生长和转移相关性状，而沉默TDRG 1导致相反的结果。我们进一步发现TDRG 1与miR-873- 5 p呈负相关，并正调控miR-873- 5 p的功能性靶基因肝癌衍生生长因子（HDGF）的表达。最后，lncRNA TDRG 1通过调节miR-873- 5 p/HDGF通路来调节GC的进展。综上所述，我们的数据揭示了TDRG 1-miR-873- 5 p-HDGF轴在人胃癌中的关键功能。
Gastric carcinoma (GC) is still one of the most common digestive system neoplasms and the primary reason for malignant cancer-associated death. Long non-coding RNAs (lncRNAs) have been reported to play critical roles in GC progression. In this study, we demonstrated that lncRNA testis development-related gene 1 (TDRG1) is markedly upregulated in clinical GC tissues and GC cells. High level of lncRNA TDRG1 correlates with the metastasis and prognosis of patients with GC. Overexpression of lncRNA TDRG1 promotes GC growth and metastatic-related traits in vitro and in vivo, and silencing TDRG1 causes opposite results. We future find that TDRG1 is inversely associated with miR-873-5p and positively modulates the expression of hepatoma-derived growth factor (HDGF), a functional target gene of miR-873-5p. Finally, lncRNA TDRG1 regulates the progression of GC through regulating miR-873-5p/HDGF pathway. Taken together, our data uncover the crucial function of TDRG1-miR-873-5p-HDGF axis in human gastric cancer.