CYTOGENETIC EVIDENCE FOR THE ABSENCE OF AN INACTIVATED X-CHROMOSOME IN A HUMAN FEMALE (XX) BREAST-CARCINOMA CELL-LINE
CYTOGENETIC EVIDENCE FOR THE ABSENCE OF AN INACTIVATED X-CHROMOSOME IN A HUMAN FEMALE (XX) BREAST-CARCINOMA CELL-LINE
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DOI：
10.1007/bf00329131
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发表时间：
1980-01-01
期刊：
影响因子：
5.3
通讯作者：
WANG, N
中科院分区：
文献类型：
--
作者：
CAMARGO, M;WANG, N
将巴尔体染色程序直接应用于女性羊膜细胞、WI 38成纤维细胞、正常女性肾细胞和人乳腺癌Elco细胞的室载玻片培养物。在所有正常雌性对照细胞中发现高频率的巴尔小体;在Elco细胞中未观察到巴尔小体。胰蛋白酶G显带分析显示，所有Elco细胞均存在2条正常X染色体。然后用末端BrdU [溴脱氧尿苷]脉冲法研究细胞系的晚期DNA复制模式。Elco细胞系中的两条X染色体均为常染色质，具有特征性的R-带型;未观察到晚复制X染色体。巴尔体和晚复制杂环X染色体的缺失为携带2条X染色体的人乳腺癌细胞中不存在失活X染色体提供了强有力的细胞遗传学证据。
Barr body staining procedures were applied directly to the chamber slide cultures of female amniotic cells, WI38 fibroblasts, normal female kidney cells and human breast carcinoma Elco cells. A high frequency of Barr bodies was found in all the normal female control cells; no Barr bodies were observed in the Elco cells. By trypsin G-banding analysis, 2 normal X chromosomes were identified in all Elco cells. The late DNA replication pattern of the cell line was then studied with the terminal BrdU [bromodeoxyuridine] pulse method. Both X chromosomes in the Elco cell line were euchromatic with a characteristic R-banding pattern; no late-replicating X chromosome was observed. The absence of both a Barr body and a late-replicating heterocyclic X chromosome provides strong cytogenetic evidence that an inactivated X chromosome is absent in the human breast carcinoma cells bearing 2 X chromosomes.