MOVEMENT OF THE X-CHROMOSOME IN EPILEPSY
MOVEMENT OF THE X-CHROMOSOME IN EPILEPSY
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DOI：
10.1126/science.3201257
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发表时间：
1988-12-23
期刊：
影响因子：
56.9
通讯作者：
MANUELIDIS, L
中科院分区：
文献类型：
--
作者：
BORDEN, J;MANUELIDIS, L
选择染色体的位置进行了评估，在正常和癫痫的人大脑皮层与生物素标记的探针特异性个别染色体域的样品。光学切片提供了一种快速的方法，在间期细胞的原位杂交信号的三维分辨率，并从数字化图像重建的详细旋转研究的固体模型。在电生理学定义的癫痫灶中，男性和女性的神经元中的X染色体发生了戏剧性的重新定位。其他染色体（1，9和Y）显示出更微妙的位置变化。涉及X染色体的特定改变的核模式可能会建立起来，并为顽固性癫痫发作活动创造遗传记忆。
The position of selected chromosomes was assessed in samples of normal and epileptic human cortex with biotinylated probes specific for individual chromosome domains. Optical sectioning provided a rapid method for three-dimensional resolution of in situ hybridization signals in interphase cells, and solid models were reconstructed from digitized images for detailed rotational studies. There was a dramatic repositioning of the X chromosome in neurons of both males and females in electrophysiologically defined seizure foci. Other chromosomes (1, 9 and Y) showed more subtle positional changes. Specifically altered nuclear patterns involving the X chromosome may become established and create the genetic memory for intractable seizure activity.