COMPARATIVE STUDIES ON RELATED FREE-LIVING AND PATHOGENIC AMEBAE WITH SPECIAL REFERENCE TO ACANTHAMOEBA
COMPARATIVE STUDIES ON RELATED FREE-LIVING AND PATHOGENIC AMEBAE WITH SPECIAL REFERENCE TO ACANTHAMOEBA
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DOI：
10.1111/j.1550-7408.1975.tb05860.x
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
BALAMUTH, W
中科院分区：
文献类型：
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作者：
VISVESVARA, GS;BALAMUTH, W
本文对不同来源的阿米巴原虫的结构、营养、蛋白质组成、免疫学及对细胞培养的影响等方面进行了比较研究。（Lilly A‐1菌株）、A. castellanii（Singh和Neff株）、A. astronyxis，A. comandoni，A. polyphaga，多食草A. Lilly A‐1菌株的阿米巴由于其对实验动物的致病性而受到特别关注。在结构、营养和抗原组成上有明显差异。和Hartmannella，认为它们是独立的属是合理的。terricola，根据孢囊结构可将所有种类的阿米巴区分开来。A. terricolaclosely相似的那些ofA.经凝胶扩散和免疫电泳（IEP）分析，证实2种阿米巴属同一种阿米巴。Lilly株与非致病性A. castellanii-Singh菌株在（a）孢囊结构;（B）蛋白质分布模式（圆盘电泳）;（c）可溶性和颗粒性抗原（d）在哺乳动物单层细胞培养物中诱导无细胞空斑和其他细胞病变效应（CPE）的能力;（e）将导致一些CPE的磷脂酶消除到培养基中，阿米巴原虫。Lilly菌株，释放更多的磷脂酶，产生更多的CPE。Lilly株在孢囊结构和抗原组成上也不同于该属其它种。因此，根据Singh和Das的建议，它应该被放在一个单独的物种中，即Culbertsoni阿米巴。
Comparative studies were conducted on the structure, nutrition, protein composition, immunology, and effect on cell cultures ofAcanthamoebasp. (Lilly A‐1 strain),A. castellanii(Singh and Neff strains),A. astronyxis, A. comandoni, A. polyphaga, A. terricola, Hartmannella vermiformis, andNaegleria gruberi.Lilly A‐1 strain ofAcanthamoebareceived special attention owing to its pathogenicity for experimental animals. Distinct differences were noted in structure, nutrition, and antigenic composition ofAcanthamoebaspp. andHartmannella, and it was concluded that their recognition as separate genera is justified.With the exception ofA. terricola, all species ofAcanthamoebacould be differentiated by cyst structure. Cysts ofA. terricolaclosely resembled those ofA. castellaniiSingh strain, and close antigenic relationships between these 2 species were demonstrated by gel diffusion and immunoelectrophoresis (IEP); it was concluded that the 2 amebae belong in the same species.The pathogenicAcanthamoebasp. Lilly strain differed from the nonpathogenicA. castellaniiSingh strain in (a) cyst structure; (b) protein distribution patterns (on disc electrophoresis); (c) soluble and particulate antigens (on gel diffusion, IEP, complement fixation, and immobilization tests); (d) capacity to induce cell‐free plaques and other cytopathic effects (CPE) in mammalian monolayer cell cultures; (e) elimination of a phospholipase, responsible for some of the CPE, into the culture medium,Acanthamoebasp. Lilly strain, which liberated more phospholipase, produced more CPE.Acanthamoebasp. Lilly strain differed also from other species of this genus in cyst structure and antigenic composition. It was concluded, therefore, that, following the recommendation of Singh & Das, it ought to be placed in a separate species,Acanthamoeba culbertsoni.