Sodium Green as a potential probe for intracellular sodium imaging based on fluorescence lifetime.
Sodium Green as a potential probe for intracellular sodium imaging based on fluorescence lifetime.
复制标题
钠绿作为基于荧光寿命的细胞内钠成像的潜在探针。
DOI：
10.1006/abio.1997.2203
复制
发表时间：
1997
影响因子：
2.9
通讯作者：
Lakowicz,JR
中科院分区：
文献类型：
--
作者：
Szmacinski,H;Lakowicz,JR
我们使用频域相位调制荧光法测量细胞内游离钠的荧光探针钠绿。发现强度衰减强烈依赖于 Na+，平均寿命从不存在 Na+ 时的 1.13 ns 增加到存在 140 mmNa+ 时的 2.39 ns。提供了浓度范围为 0 至 500 mm 的 Na+ 和 K+ 存在下强度衰减的详细分析。描述了使用在单一调制频率下测量的数据进行钠感测。相位和调制数据显示对 Na+ 具有高灵敏度，而对 K+ 则显着降低灵敏度。此外，将钠绿暴露在强光照下表明钠绿比其前体荧光素具有更高的光稳定性。这些结果表明，基于寿命的钠绿测量可用于对约 0.5 至 50 mm 范围内的细胞内游离 [Na+] 进行高精度成像。
We characterized the use of the fluorescent probe Sodium Green for measurements of intracellular free sodium using frequency–domain, phase–modulation fluorometry. The intensity decays were found to be strongly Na+dependent, with mean lifetime increasing from 1.13 ns in the absence of Na+to 2.39 ns in the presence of 140 mmNa+. Detailed analysis of the intensity decays in the presence of Na+and K+in the concentration range from 0 to 500 mmis provided. Sodium sensing using data measured at a single modulation frequency is described. Phase and modulation data showed high sensitivity to Na+and substantially lower sensitivity to K+. Additionally, exposure of Sodium Green to intense illumination indicated that Sodium Green is much more photostable than its precursor, fluorescein. These results indicate that lifetime-based measurements with Sodium Green can be used for imaging of intracellular free [Na+] in the range from about 0.5 to 50 mmwith high accuracy.