Design of an implantable artificial dural window for chronic two-photon optical imaging in non-human primates.
Design of an implantable artificial dural window for chronic two-photon optical imaging in non-human primates.
复制标题
用于非人类灵长类动物慢性双光子光学成像的植入式人工硬脑膜窗的设计。
DOI：
10.1109/embc.2015.7320140
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Shenoy,Krishna
中科院分区：
文献类型：
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作者：
Trautmann,Eric;O'Shea,DanielJ;Shrestha,Shikhar;Lin,Steven;Ryu,Stephen;Shenoy,Krishna
钙离子成像等光学功能成像方法已成为研究体内神经活动的有力工具。在这里，我们提出了一种钛植入室的设计，它带有透明的硅胶人造硬脑膜，可以在非人类灵长类动物中进行双光子钙成像。这个小室使用高数值孔径多光子物镜进行成像，并保持密封，保护大脑免受周围环境的影响。此外，我们描述了一种可调的组织稳定系统，在成像过程中施加温和的机械压力来稳定组织。我们的结果表明，双光子钙成像可能很快就会促进在非人类灵长类动物中进行一类新的电路和系统神经科学实验。
Optical functional imaging methods such as calcium imaging have become a powerful tool for investigating neural activity in-vivo. Here, we present a design for a titanium implantable chamber with transparent silicone artificial dura which enables two-photon calcium imaging in non-human primates. This chamber accommodates imaging with high numerical aperture multiphoton objective lenses, and remains sealed, protecting the brain from the surrounding environment. In addition, we describe a tunable tissue stabilization system to apply gentle mechanical pressure to stabilize tissue during imaging. Our results suggest that two-photon calcium imaging may soon facilitate a new class of circuit and systems neuroscience experiments in non-human primates.