Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment.
Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment.
复制标题
与温带沿海环境中浮游植物大量繁殖相关的活性海洋甲基营养菌的底物特异性分支。
DOI：
10.1128/aem.01266-08
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发表时间：
2008
影响因子：
4.4
通讯作者：
Neufeld JD
中科院分区：
文献类型：
--
作者：
Neufeld JD
消耗一碳（C1）化合物的海洋微生物的描述很少，尽管它们通过影响水生和大气化学对全球气候产生影响。本研究调查了参与C1化合物代谢的海洋细菌群落。这些社区是相关的表层海水和大气化学的背景下，主要是浮游植物已知生产二甲基磺基丙酸盐的水华。除了使用16 S rRNA基因指纹图谱和克隆库，以表征从2006年7月的水华样带，从浮游植物水华的海水样品进行了孵育与13 C标记的甲醇，一甲胺，二甲胺，溴甲烷，二甲基硫醚，以确定微生物种群参与C1化合物的营业额，使用DNA稳定同位素探测。采用变性梯度凝胶电泳（DGGE）、指纹聚类分析和16 S rRNA基因克隆文库分析对单个时间点的[13 C]DNA样品进行表征和比较。细菌群落DGGE指纹从13 C-标记的DNA是不同的，从那些获得的非标记的社区DNA的DNA，并建议在不同的C1基板上生长的活性甲基营养菌种群之间的基板利用的一些重叠。活跃的甲基营养菌隶属于嗜甲基菌属。还有几个未描述的γ-变形菌分支，它们利用甲醇、甲胺（一甲胺和二甲胺）和二甲硫醚。与同化13 C标记的溴甲烷和其他底物的群体相对应的rRNA基因序列与α变形菌门的成员（例如，杜鹃花科）、噬细胞-嗜纤维杆菌-拟杆菌群和未知分类群。这项研究扩展了已知的海洋甲基营养菌在表层海水中的多样性，并为未来的重点培养和宏基因组分析提供了全面的数据集。
Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry. This study investigated marine bacterial communities involved in the metabolism of C1compounds. These communities were of relevance to surface seawater and atmospheric chemistry in the context of a bloom that was dominated by phytoplankton known to produce dimethylsulfoniopropionate. In addition to using 16S rRNA gene fingerprinting and clone libraries to characterize samples taken from a bloom transect in July 2006, seawater samples from the phytoplankton bloom were incubated with13C-labeled methanol, monomethylamine, dimethylamine, methyl bromide, and dimethyl sulfide to identify microbial populations involved in the turnover of C1compounds, using DNA stable isotope probing. The [13C]DNA samples from a single time point were characterized and compared using denaturing gradient gel electrophoresis (DGGE), fingerprint cluster analysis, and 16S rRNA gene clone library analysis. Bacterial community DGGE fingerprints from13C-labeled DNA were distinct from those obtained with the DNA of the nonlabeled community DNA and suggested some overlap in substrate utilization between active methylotroph populations growing on different C1substrates. Active methylotrophs were affiliated withMethylophagaspp. and several clades of undescribedGammaproteobacteriathat utilized methanol, methylamines (both monomethylamine and dimethylamine), and dimethyl sulfide. rRNA gene sequences corresponding to populations assimilating13C-labeled methyl bromide and other substrates were associated with members of theAlphaproteobacteria(e.g., the familyRhodobacteraceae), theCytophaga-Flexibacter-Bacteroidesgroup, and unknown taxa. This study expands the known diversity of marine methylotrophs in surface seawater and provides a comprehensive data set for focused cultivation and metagenomic analyses in the future.