Nebulin isoforms of extraocular muscle.
Nebulin isoforms of extraocular muscle.
复制标题
眼外肌的星云蛋白亚型。
DOI：
10.1007/s00441-006-0311-4
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发表时间：
2007
影响因子：
3.6
通讯作者：
Andrade,FranciscoH
中科院分区：
文献类型：
--
作者：
Moncman,CaroleL;Andrade,FranciscoH
眼外肌（EOM）负责反射性和自主性眼球运动，具有许多独特的生化，生理和超微结构特征，使其与其他骨骼肌区分开来。例如，啮齿动物EOM缺乏M系，并表达EOM特异性肌球蛋白重链（MYH 13）和α-心肌肌球蛋白重链。最近的基因表达谱研究表明，在成人眼外肌中存在其他心脏特异性蛋白。这种有趣的混合物的肌原纤维和细胞骨架蛋白提出的问题，是否nebulette，而不是nebulin，可能会在EOM中表达，什么样的肌联蛋白亚型在EOM中表达。我们已经进行了凝胶电泳和免疫分析，以确定肌联蛋白和星云蛋白亚型表达的眼外肌。我们已经发现，在眼外肌中表达的肌联蛋白同种型的质量最接近于在测试的骨骼肌中发现的质量，即，比目鱼肌和趾长伸肌（EDL）。我们还表明，虽然EOM表达心肌肌球蛋白的亚型，它不表达nebulette和包含一个星云蛋白亚型的质量与原型快速后肢肌肉EDL中发现的一致。
The extraocular muscles (EOMs), which are responsible for reflexive and voluntary eye movements, have many unique biochemical, physiological, and ultrastructural features that set them apart from other skeletal muscles. For example, rodent EOMs lack M-lines and express EOM-specific myosin heavy chain (MYH13) and α-cardiac myosin heavy chain. Recent gene-expression profiling studies indicate the presence of other cardiac-specific proteins in adult EOMs. This interesting mixture of myofibrillar and cytoskeletal proteins poses the questions as to whether nebulette, as opposed to nebulin, might be expressed in EOM, and what isoforms of titin are expressed in the EOM. We have performed gel electrophoresis and immunological analyses to determine the titin and nebulin isoforms expressed in the EOM. We have found that the mass of the titin isoforms expressed in the EOM most closely resemble those found in the skeletal muscles tested, viz., the soleus and extensor digitorum longus (EDL). We also demonstrate that, although the EOM expresses cardiac isoforms of myosin, it does not express nebulette and contains a nebulin isoform with a mass consistent with that found in the prototypical fast hindlimb muscle EDL.