Non-contact protein microarray fabrication using a procedure based on liquid bridge formation
Non-contact protein microarray fabrication using a procedure based on liquid bridge formation
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DOI：
10.1007/s00216-008-2509-7
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Roeraade, Johan
中科院分区：
文献类型：
--
作者：
Hartmann, Michael;Sjodahl, Johan;Roeraade, Johan
目前用于蛋白质微阵列制造的接触式或非接触式微阵列仍然存在许多问题，例如产生卫星斑点，不均匀斑点，错位甚至缺失斑点，以及样品携带。本文提出了一种新的非接触样品沉积的概念，以减少这类问题。为了展示这种压力辅助沉积技术的潜力和稳健性，我们精确地打印了已知会导致严重问题或甚至无法用传统微阵列打印的不同样品溶液。样品包括200 mg mL（-1）人血清白蛋白、高浓度粘性细胞黏附蛋白、纯高盐细胞裂解缓冲液、纯DMSO和5 μ m聚苯乙烯微球悬浮液。此外，一种与水不混溶的液态碳氟化合物被用作盖子，以减少微阵列打印过程中的蒸发，这种液体碳氟化合物被证明不会影响捕获分子的功能。氟碳液体盖子在长期沉积过程中可以避免水敏感活化表面的水解。
Contemporary microarrayers of contact or noncontact format used in protein microarray fabrication still suffer from a number of problems, e. g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL(-1) human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-mu m polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.