CD47 protein expression in acute myeloid leukemia: A tissue microarray-based analysis
CD47 protein expression in acute myeloid leukemia: A tissue microarray-based analysis
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DOI：
10.1016/j.leukres.2015.04.007
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发表时间：
2015-07-01
影响因子：
2.7
通讯作者：
Banz, Yara
中科院分区：
文献类型：
--
作者：
Galli, Serena;Zlobec, Inti;Banz, Yara
CD 47与信号调节蛋白α（SIRP α）（一种抑制性受体）的结合负调节吞噬作用。在急性髓系白血病（AML）中，CD 47在外周血原始细胞和白血病干细胞上过度表达，与生存率呈负相关，本研究的目的是通过免疫组织化学（IHC）在骨髓（BM）组织芯片（TMA）中的CD 47蛋白表达与AML患者临床结局之间的相关性。对BM白血病原始细胞上的CD 47染色进行半定量评分，并与AML的临床参数和已知预后因素相关。在75%和25%的AML患者中观察到低（评分0-2）和高（评分3）CD 47蛋白表达。CD 47表达与BM原始细胞浸润百分比和外周血原始细胞百分比显著相关。此外，高CD 47表达与核磷蛋白（NPM 1）基因突变有关。相比之下，CD 47表达与总体或无进展生存期或对治疗的反应无显著相关性。总之，BM TMA允许通过IHC对CD 47蛋白表达进行快速和可重复的半定量分析。虽然循环AML原始细胞上的CD 47表达已被证明是非常明确的NK AML AML患者人群的阴性预后标志物，但AML BM原始细胞上的CD 47表达不是。(C)2015爱思唯尔有限公司版权所有。
Binding of CD47 to signal regulatory protein alpha (SIRP alpha), an inhibitory receptor, negatively regulates phagocytosis. In acute myeloid leukemia (AML), CD47 is overexpressed on peripheral blasts and leukemia stem cells and inversely correlates with survival.Aim of the study was to investigate the correlation between CD47 protein expression by immunohistochemistry (IHC) in a bone marrow (BM) tissue microarray (TMA) and clinical outcome in AML patients. CD47 staining on BM leukemia blasts was scored semi-quantitatively and correlated with clinical parameters and known prognostic factors in AML. Low ( scores 0-2) and high (score 3) CD47 protein expression were observed in 75% and 25% of AML patients. CD47 expression significantly correlated with percentage BM blast infiltration and peripheral blood blasts. Moreover, high CD47 expression was associated with nucleophosmin (NPM1) gene mutations. In contrast, CD47 expression did not significantly correlate with overall or progression free survival or response to therapy.In summary, a BM TMA permits rapid and reproducible semi-quantitative analysis of CD47 protein expression by IHC. While CD47 expression on circulating AML blasts has been shown to be a negative prognostic marker for a very defined population of AML patients with NK AML, CD47 expression on AML BM blasts is not. (C) 2015 Elsevier Ltd. All rights reserved.