Stress neuropeptides evoke epithelial responses via mast cell activation in the rat colon
Stress neuropeptides evoke epithelial responses via mast cell activation in the rat colon
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DOI：
10.1016/j.psyneuen.2008.07.002
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发表时间：
2008-10-01
影响因子：
3.7
通讯作者：
Perdue, Mary H.
中科院分区：
文献类型：
--
作者：
Santos, Javier;Yates, Derrick;Perdue, Mary H.
背景资料：以前，我们发现，促肾上腺皮质激素释放因子（CRF）腹腔注射模仿上皮细胞对应激的反应，刺激离子分泌和增加大鼠结肠的通透性，肥大细胞参与其中。然而，CRF敏感的粘膜/粘膜下环的能力，以调节肠屏障和参与居民mast cells是uncleared.Methods：我们检查结肠上皮[的反应，应激样肽在Wistar-Kyoto（WKY），和肥大细胞缺陷（Ws/Ws）和他们的+/+同窝对照大鼠在远端段安装在Ussing chamber。短路电流（离子分泌），辣根过氧化物酶通量在用astressin、doxantrazole或赋形剂预处理的组织中，测量CRF [10（-6）至10（-8）M]或sauvagine [10（-8）至10（-10）M]对大鼠肥大细胞蛋白酶11的释放的响应。应激样肽（sauvagine > CRF）诱导WKY中短路电流的剂量依赖性增加（在30 min时最大），并显著增强辣根过氧化物酶通量和蛋白酶11释放。astressin和doxantrazole抑制上皮细胞的反应，并显着减少在组织从Ws/Ws rats.Conclusion：应激介质CRF和sauvagine调节屏障功能在大鼠结肠粘膜/粘膜下CRF受体轴承细胞，通过肥大细胞依赖的途径。(C)2008爱思唯尔有限公司保留所有权利。
Background: Previously, we showed that corticotropin-releasing factor (CRF) injected i.p. mimicked epithelial responses to stress, both stimulating ion secretion and enhancing permeability in the rat colon, and mast cells were involved. However, the ability of CRF-sensitive mucosal/submucosal Loops to regulate intestinal barrier and the participation of resident mast cells are unclear.Methods: We examined colonic epithelia[ responses to stress-Like peptides in Wistar-Kyoto (WKY), and mast cell-deficient (Ws/Ws) and their +/+ littermate control rats in distal segments mounted in Ussing chambers. Short-circuit current (ion secretion), flux of horseradish peroxidase (macromolecular permeability), and the release of rat mast cell protease 11 were measured in response to CRF [10(-6) to 10(-8) M] or sauvagine [10(-8) to 10(-10) M] in tissues pretreated with astressin, doxantrazole, or vehicle.Results: Stress-Like peptides (sauvagine > CRF) induced a dose-dependent increase in short-circuit current (maximal at 30 min), and significantly enhanced horseradish peroxidase flux and protease 11 release in WKY. Epithelial responses were inhibited by both astressin and doxantrazole, and significantly reduced in tissues from Ws/Ws rats.Conclusion: The stress mediators CRF and sauvagine modulate barrier function in the rat colon acting on mucosal/submucosal CRF receptor-bearing cells, through mast cell-dependent pathways. (C) 2008 Elsevier Ltd. All rights reserved.