Intermediate filament proteins in the developing chick spinal cord.
Intermediate filament proteins in the developing chick spinal cord.
复制标题
发育中的小鸡脊髓中的中间丝蛋白。
DOI：
10.1016/0012-1606(81)90313-4
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Holtzer,H
中科院分区：
文献类型：
--
作者：
Tapscott,SJ;Bennett,GS;Toyama,Y;Kleinbart,F;Holtzer,H
使用免疫组织化学技术、分析凝胶电泳和电子显微镜检查了胚胎鸡脊髓中不同发育阶段的不同中间丝 (IF) 蛋白的分布。我们发现：（1）成纤维细胞型IF蛋白（波形蛋白）几乎存在于早期神经管的所有复制神经上皮细胞中，以及发育后期的放射状胶质细胞、星形胶质细胞和雪旺细胞中； (2)在定形神经元中检测不到成纤维细胞型IF蛋白； (3)神经丝蛋白首先在有丝分裂后神经母细胞中大约在初始轴突形成时被检测到，并且仅限于神经元； (4)星形胶质细胞型IF蛋白(胶质纤维酸性蛋白)存在于定型星形胶质细胞中，但不存在于放射状胶质细胞中； (5)前角蛋白仅限于软脑膜细胞； (6) 肌肉型 IF 蛋白（结蛋白）仅限于发育中的脊髓内部和周围的血管组织。这些发现表明，成纤维细胞型 IF 蛋白是早期神经上皮细胞中唯一的 IF 蛋白，并且这些细胞的后代将遵循 IF 蛋白表达的三种不同模式之一：（1）仅持续表达成纤维细胞型 IF 蛋白（放射状胶质细胞）； (2)成纤维细胞型IF蛋白和星形胶质细胞型IF蛋白(星形胶质细胞)两者的表达；或（3）仅表达神经丝蛋白（神经元）。
The distribution of different intermediate filament (IF) proteins in the embryonic chick spinal cord was examined at several stages of development using immunohistochemical techniques, analytic gel electrophoresis, and electron microscopy. We have found that: (1) the fibroblast-type IF protein (vimentin) is present in virtually all of the replicating neuroepithelial cells of the early neural tube, as well as in radial glia, astrocytes, and Schwann cells in later stages of development; (2) the fibroblast-type IF protein is not detectable in definitive neurons; (3) the neurofilament proteins are first detectable in postmitotic neuroblasts at about the time of initial axon formation and they are restricted to neurons; (4) the astrocyte-type IF protein (glial fibrillary acidic protein) is in definitive astrocytes, but not in radial glia; (5) the prekeratin proteins are restricted to cells of the leptomeninges; and (6) the muscle-type IF protein (desmin) is restricted to vascular tissue in and around the developing spinal cord. These findings suggest that the fibroblast-type IF protein is the only IF protein in the early neuroepithelial cells and that the progeny of these cells will follow one of three different patterns of IF protein expression: (1) continued expression of only the fibroblast-type IF protein (radial glia); (2) expression of both the fibroblast-type IF protein and the astrocyte-type IF protein (astrocytes); or (3) expression of only the neurofilament proteins (neurons).