1-Methyladenine can consistently induce a fura-detectable transient calcium increase which is neither necessary nor sufficient for maturation in oocytes of the starfish Asterina miniata.
1-Methyladenine can consistently induce a fura-detectable transient calcium increase which is neither necessary nor sufficient for maturation in oocytes of the starfish Asterina miniata.
复制标题
1-甲基腺嘌呤可以持续诱导可检测到的瞬时钙增加，这对于海星海星卵母细胞的成熟既不是必要的也不是充分的。
DOI：
10.1016/0012-1606(90)90393-w
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发表时间：
1990
影响因子：
2.7
通讯作者：
Steinhardt,RA
中科院分区：
文献类型：
--
作者：
Witchel,HJ;Steinhardt,RA
在海星卵母细胞中，可以用染料 fura-2 一致地检测到响应 1-甲基腺嘌呤（负责诱导减数分裂成熟的激素）阈值水平灌注的钙瞬变。当激素缓慢扩散至卵母细胞时，无法检测到钙瞬变，并且可以通过用低于阈值水平的激素对卵母细胞进行预灌注来抑制激素诱导的钙瞬变。通过在与钙瞬变无关的情况下引发成熟，表明钙瞬变对于成熟来说是不必要的，并且通过引发钙瞬变并在激素依赖期结束之前将激素从卵母细胞上洗掉，钙瞬变对于成熟来说是不够的。有人提出了一种机制来解释为什么一些研究人员在卵母细胞成熟诱导过程中检测到短暂的钙升高，而另一些研究人员却没有。
In starfish oocytes a calcium transient can consistently be detected with the dye fura-2 in response to perfusion of threshold levels of 1-methyladenine, the hormone responsible for induction of meiotic maturation. The calcium transient cannot be detected when the hormone is allowed to slowly diffuse to the oocyte, and the hormone-induced calcium transient can be inhibited by preperfusion of the oocyte with subthreshold levels of hormone. The calcium transient is shown to be unnecessary for maturation by eliciting maturation in situations that are not associated with a calcium transient, and the calcium transient is shown to be insufficient for maturation by eliciting a calcium transient and washing the hormone off the oocytes before the end of the hormone-dependent period. A mechanism is suggested to explain why some investigators have detected transient calcium rises during induction of oocyte maturation while others have not.