Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii
Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii
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DOI：
10.1007/s12041-015-0559-0
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发表时间：
2015-09-01
影响因子：
1.5
通讯作者：
Cui, Dang Qun
中科院分区：
文献类型：
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作者：
Dong, Zhong Dong;Chen, Jie;Cui, Dang Qun
为研究中国小麦Myb10-1基因的等位变异及其与小麦发芽水平的关系，对582个中国面包小麦品种和110份节节麦材料进行了鉴定，鉴定了3个Myb10-1基因的等位变异。鉴定结果表明，5个中国地方品种中存在一个新的Tamyb10-B1等位基因，命名为Tamyb10-B1c。 Tamyb10-B1c 可能存在包括 Tamyb10-B1 基因在内的大缺失。在节节山羊草中发现了三个新的 Tamyb10-D1 等位基因（Aetmyb10-D1c、Aetmyb10-D1d 和 Aetmyb10-D1e）。其中，Aetmyb10-D1c 等位基因具有 104 bp 缺失，这导致 Aetmyb10-D1 基因开放阅读框发生移码。与 TAMYB10-D1b 蛋白相比，AETMYB10-D1d 和 AETMYB10-D1e 蛋白分别具有三个和两个不同的氨基酸。 Tamyb10-1等位基因变异与籽粒发芽水平的关联表明，与白粒Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1a基因型相比，红色籽粒的所有5个等位基因组合在储藏一年后均表现出显着更高的GP（发芽率）和GI（发芽指数）值。此外，Tamyb10-A1b/Tamyb10-B1c/Tamyb10-D1b 基因型在六种不同的 Tamyb10-1 组合中具有显着最高的 GP 和 GI。这项研究可以为小麦育种计划在籽粒颜色和发芽水平方面提供有用的信息。
To investigate allelic variation of Myb10-1 genes in Chinese wheat and to examine its association with germination level in wheat, a total of 582 Chinese bread wheat cultivars and 110 Aegilops tauschii accessions were used to identify allelic variations of three Myb10-1 genes. Identification results indicated that there is a novel Tamyb10-B1 allele, designated Tamyb10-B1c, in the five Chinese landraces. The Tamyb10-B1c possibly has a large deletion including Tamyb10-B1 gene. There are three novel Tamyb10-D1 alleles (Aetmyb10-D1c, Aetmyb10-D1d and Aetmyb10-D1e) that were discovered in Aegilops tauschii.. Of them, Aetmyb10-D1c allele possessed a 104-bp deletion and this resulted in a frame shift in the open reading frame of the Aetmyb10-D1 gene. AETMYB10-D1d and AETMYB10-D1e proteins possessed three and two different amino acids when compared with TAMYB10-D1b protein, respectively. Association of Tamyb10-1 allelic variation with grain germination level indicated that all five allelic combinations with red grains showed a significantly higher GP (germination percentage) and GI (germination index) values than those of white-grained Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1a genotype after storing it for one year. Moreover, the Tamyb10-A1b/Tamyb10-B1c/Tamyb10-D1b genotype possesses the significantly highest GP and GI among the six different Tamyb10-1 combinations. This study could provide useful information for wheat breeding programme in terms of grain colour and germination level.