EVALUATION OF SERUM AMYLOID-A PROTEIN AS AN ACUTE-PHASE REACTIVE PROTEIN IN HORSES
EVALUATION OF SERUM AMYLOID-A PROTEIN AS AN ACUTE-PHASE REACTIVE PROTEIN IN HORSES
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DOI：
10.1292/jvms.55.1011
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发表时间：
1993-12-01
影响因子：
1.2
通讯作者：
HAGIO, M
中科院分区：
文献类型：
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作者：
NUNOKAWA, Y;FUJINAGA, T;HAGIO, M
用SephadexG-75凝胶过滤3次，从马急性期血清中分离出血清淀粉样蛋白A（SAA）。用兔抗马SAA血清，采用单向免疫扩散技术对马SAA进行定量检测。在临床正常的马中，SAA浓度从出生后立即到1周龄保持相对较高。此后，浓度在约13至30 μ g/ml范围内显示周期性波动。马驹（小于或等于12月龄）和成年马（大于或等于18月龄）中SAA的平均（+/- SD）浓度分别为19.37 +/- 9.41和21.53 +/- 9.81 μ g/ml。在围生期妊娠中，SAA浓度在分娩前4个月内保持稳定并在正常范围内。产驹后迅速升高，产后第3天达峰值136.78 ± 56.74 μ g/ml，产后2周开始下降，产后1个月恢复到正常范围。在实验诱导炎症的马中，SAA浓度迅速增加，并在给药后第2天达到最高值，约为给药前值的4至20倍。然后在10天至4周内恢复到基线值，同时局部炎症体征消失。SAA浓度在大多数具有炎症临床体征的马中非常高。结果表明，马SAA是一种敏感的急性时相反应蛋白，在各种急性炎症的早期即升高。
Serum amyloid A protein (SAA) was isolated from equine acute-phase serum by repeating Sephadex G-75 gel filtration 3 times. Quantitative measurement of equine SAA was performed by the single radial immunodiffusion technique with rabbit anti-equine SAA serum. In clinically normal horses, the SAA concentration remained relatively high from immediately after birth up to 1 week of age. After this the concentration showed periodic fluctiation in the range of approximately 13 to 30 mu g/ml. The mean (+/- SD) concentration of SAA in foals (less than or equal to 12 months old) and in adult horses (greater than or equal to 18 months old) was 19.37 +/- 9.41 and 21.53 +/- 9.81 mu g/ml, respectively. In mares during the perinatal period, the SAA concentration remained stable and within the normal range for 4 months before parturition. After foaling, it increased quickly and reached a peak value of 136.78 +/- 56.74 mu g/ml on day 3 postpartum, and then began to decrease at 2 weeks postpartum returning to within the normal range by I month postpartum. In horses with experimentally induced inflammation, the SAA concentration increased quickly, and reached the highest value, approximately 4 to 20 times higher than pre-treatment values, on day 2 after treatment. It then returned to the base line values within IO days to 4 weeks, concurrent with the disappearance of local inflammatory signs. The SAA concentration was very high in most horses with clinical signs of inflammation. It was concluded from these data that equine SAA was a sensitive acute-phase reactive protein which increased in the early phase of various acute inflammations.