Use of PC12 cells and rat superior cervical ganglion sympathetic neurons as models for neuroprotective assays relevant to Parkinson's disease.
Use of PC12 cells and rat superior cervical ganglion sympathetic neurons as models for neuroprotective assays relevant to Parkinson's disease.
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DOI：
10.1007/978-1-61779-536-7_18
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Greene, Lloyd A
中科院分区：
文献类型：
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作者：
Grau, Cristina Malagelada;Greene, Lloyd A
由原代神经元培养物或神经元样细胞系组成的细胞模型通常用于研究神经元细胞死亡和测试特定化合物的神经保护性质。细胞模型很容易获得，允许解剖和调节参与神经元死亡的信号通路。例如，药物或shRNA递送更直接，因为没有血脑屏障可供穿越。然而，由于这些模型有其局限性，任何重要的发现最终都应该用动物模型和人体样本进行验证。在这里，我们描述了两个细胞模型，可以用作一个高度信息化和易于使用的起点，用于测试潜在的神经保护药物帕金森病：PC12细胞和交感神经元细胞培养。我们详细描述了应用这些模型研究帕金森病背景下的神经保护所需的方案。
Cellular models composed of primary neuronal cultures or neuron-like cell lines are commonly used to study neuron cell death and to test the neuroprotective properties of specific compounds. Cellular models are easily accessible, permitting dissection and modulation of signaling pathways involved in neuron death. For example, drug or shRNA delivery is more straightforward since there is no blood–brain barrier to cross. However, since these models have their limitations, any important findings should ultimately be verified with animal models and human samples. Here, we describe two cellular models that can be used as a highly informative and easy to use starting point for testing potential neuroprotective drugs for Parkinson’s disease: PC12 cells and sympathetic neuronal cell cultures. We describe in detail the protocols needed to apply these models to study neuroprotection in the context of Parkinson’s disease.