Elongated cell morphology and uniaxial mechanical stretch contribute to physical attributes of niche environment for MSC tenogenic differentiation
Elongated cell morphology and uniaxial mechanical stretch contribute to physical attributes of niche environment for MSC tenogenic differentiation
复制标题
细长的细胞形态和单轴机械拉伸有助于 MSC 肌腱分化的生态位环境的物理属性
DOI：
10.1002/cbin.10094
复制
发表时间：
2013-07-01
影响因子：
3.9
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Wang, Wenbo;Deng, Dan;Liu, Wei
间充质干细胞（MSCs）的特异性谱系分化，如成骨和软骨分化，是基于MSCs的肌肉骨骼组织再生的主要课题。然而，对于间充质干细胞成腱鞘分化的理想诱导机制探索较少。虽然在过去几十年中，使用化学信号（如生长因子）诱导MSCs分化一直是主流方法，但最近的研究表明，物理信号是构成MSCs体内生态位的主要属性之一，并且在几种体外模型中是决定MSC命运的有力因素；例如，通过表面形貌控制细胞形状可以驱动间充质干细胞向特定谱系分化。机械信号在MSCs向某些谱系分化过程中也起着重要作用。肌腱是一种通过身体活动受到动态单轴机械拉伸的结缔组织。此外，腱细胞具有独特的细长细胞形态，并与胶原纤维平行排列。因此，我们假设强制延长的细胞形态和单轴机械拉伸信号都有助于肌腱细胞在体内微环境中的主要物理生态位属性，并且模仿这些物理信号可能足以诱导MSCs的肌腱分化。
Specific lineage differentiation of mesenchymal stem cells (MSCs), such as osteogenic and chondrogenic differentiation, is the major subject of MSC-based musculoskeletal tissue regeneration. Nevertheless, an ideal induction regime for MSC tenogenic differentiation is less explored. While induced differentiation of MSCs using chemical signalling, such as growth factors, has been the mainstream methodology in the past decades, recent studies show that a physical signal is one of the major attributes that composes in vivo niches of MSCs, and is a potent factor in determining MSC fate in several in vitro models; for example, control of cell shape with surface topography can drive MSCs towards a specific lineage differentiation. Mechanical signals also play important roles in the differentiation of MSCs towards certain lineages. Tendon is a connective tissue which is subjected to dynamic uniaxial mechanical stretch by physical activity. Additionally, tenocytes have a unique elongated cell morphology and are aligned in parallel with collagen fibres. We thus would hypothesize that both enforced elongated cell morphology and uniaxial mechanical stretch signal contribute to the major physical niche attributes of tenocytes' in vivo microenvironment, and mimicking these physical signals may be sufficient to induce tenogenic differentiation of MSCs.